SID1 transmembrane family, member 2 (Sidt2) A novel lysosomal membrane protein
SID1 transmembrane family, member 2 (Sidt2) A novel lysosomal membrane protein
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DOI：
10.1016/j.bbrc.2010.09.133
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发表时间：
2010-11-26
影响因子：
3.1
通讯作者：
Zhang Huiwen
中科院分区：
文献类型：
--
作者：
Gao Jialin;Gu Xuefan;Zhang Huiwen
在最近的溶酶体蛋白的蛋白质组学研究中[10]，我们鉴定了SID 1跨膜家族成员2（Sidt 2）作为新的溶酶体膜蛋白候选物。Sidt 2基因编码832个氨基酸残基的蛋白，计算的分子量为945 kDa。生物信息学分析表明，Sidt 2是一种多通道跨膜蛋白，含有10个推定的N-糖基化位点（NxS/T）。和两个潜在的基于酪氨酸的分选信号（YGSF和YDTL）使用特异性抗Sidt 2抗体和溶酶体标记物，通过免疫荧光确定Sidt 2的溶酶体定位。此外，使用亚细胞分级分离技术，我们证明Sidt 2是溶酶体整合膜蛋白。130 kDa分子量由于广泛的糖基化在用PNGase F消化后，Sidt 2的表观分子量降低至95 kDa的预测值。在大鼠中，Sidt 2在肝、脑和肾中高度表达，而在骨骼肌、心脏和其他组织中没有或几乎没有表达。特定表达（C）2010 Elsevier Inc版权所有
In a recent proteomic study of lysosomal proteins [10] we identified SID1 transmembrane family member 2 (Sidt2) as a novel lysosomal membrane protein candidate The Sidt2 gene encodes an 832-amino acid residues protein with a calculated molecular mass of 945 kDa Bioinformatic analysis showed that Sidt2 is a multipass transmembrane protein that contains 10 putative N-glycosylation sites (NxS/T) and two potential tyrosine-based sorting signals (YGSF and YDTL) Using specific anti-Sidt2 antibody and lysosomal markers the lysosomal localization of Sidt2 was determined by immunofluorescence Furthermore using subcellular fractionation techniques we demonstrated that Sidt2 is a lysosomal integral membrane protein Endogenous Sidt2 was detected in multiple tissues of mouse and rat with approximately 120-130 kDa molecular weights due to extensive glycosylation After digestion with PNGase F the apparent molecular mass of Sidt2 decreased to the predicted value of 95 kDa In rats Sidt2 was highly expressed in the liver brain and kidney whereas no or little expression was found in the skeletal muscles heart and other tissues In summary Sidt2 is a highly glycosylated lysosomal integral membrane protein that shows tissue-specific expression (C) 2010 Elsevier Inc All rights reserved