Effects of semen processing on the generation of reactive oxygen species and mitochondrial membrane potential of human spermatozoa
Effects of semen processing on the generation of reactive oxygen species and mitochondrial membrane potential of human spermatozoa
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DOI：
10.1111/j.1439-0272.2010.01123.x
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发表时间：
2012-06-01
期刊：
影响因子：
2.4
通讯作者：
Lin, Q.
中科院分区：
文献类型：
--
作者：
Li, Z.;Zhou, Y.;Lin, Q.
本研究旨在评价精液加工对精子胞内活性氧（ROS）和线粒体膜电位（MMP）产生的影响，为精子制备过程中评价精子质量提供可靠的指标。采用游泳法和密度梯度离心分离法分离少弱无精子症（OAT）组、白细胞精症（LC）组和正常精子症组的精液。流式细胞术检测ROS和MMP水平。制备前，精液参数异常患者MMP较低，ROS较高，且MMP与ROS呈负相关。MMP和ROS水平显著升高，尤其是游泳产生的ROS。OAT组制备后MMP与正常活动精子总数的相关性有显著差异。LC组ROS水平与白细胞数量相关。MMP可作为评价OAT患者精子质量的客观指标，MMP与ROS联合应用可评价LC患者精子制备效率。这些发现可以指导针对不同精子样本选择理想的精子分离技术。
This study was designed to evaluate the effects of semen processing on the generation of intracellular reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) in spermatozoa, and to develop reliable indexes for the evaluation of sperm quality during sperm preparation. Swim-up and density gradient centrifugation methods were used to separate semen in oligoasthenoteratozoospermia (OAT), leucocytospermia (LC) and normozoospermia groups. Levels of ROS and MMP were measured by flow cytometry. Before preparation, the patients with abnormal semen parameters had a lower MMP and higher ROS, and there was a negative correlation between MMP and ROS. The levels of MMP and ROS increased significantly, especially ROS produced by swim-up. A significant difference was found between the correlation of MMP and total normal motile sperm count after preparation in the OAT group. The level of ROS was associated with the amount of white blood cells in the LC group. The MMP can be used as an objective index to evaluate the sperm quality of OAT patients, and the combination of MMP and ROS can be used to assess the efficiency of sperm preparation in LC patients. These findings can guide selection of the ideal sperm separation technique for different sperm samples.