Decreased expression of pigment epithelium-derived factor and increased microvascular density in ovarian endometriotic lesions in women with endometriosis
Decreased expression of pigment epithelium-derived factor and increased microvascular density in ovarian endometriotic lesions in women with endometriosis
复制标题
子宫内膜异位症女性卵巢子宫内膜异位病变中色素上皮衍生因子表达减少和微血管密度增加。
DOI：
10.1016/j.ejogrb.2012.06.034
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发表时间：
2012-11-01
影响因子：
2.6
通讯作者：
Zhang, Xinmei
中科院分区：
文献类型：
--
作者：
Huang, Xiufeng;Chen, Liqing;Zhang, Xinmei
目的：为了确定是否有子宫内膜异位症的妇女有改变表达的色素上皮衍生因子（PEDF）在卵巢增生性病变相比，妇女没有endometriosis.Study design：异位和在位和正常子宫内膜组织分别从40名妇女卵巢子宫内膜异位症和20名对照妇女，分别。子宫内膜PEDF的表达和微血管密度（MVD）使用抗体的血管性血友病因子（vWF）和α-平滑肌肌动蛋白（α-SMA）进行了评估，通过使用免疫组化staining.Results：我们检测到减少PEDF的表达和增加微血管密度使用抗vWF和α-SMA在卵巢上皮性病变的妇女子宫内膜异位症与对照组相比。在子宫内膜异位症患者中，使用抗vWF和α-SMA而非PEDF表达的MVD与卵巢增生性囊肿的大小和疾病的严重程度相关。此外，MVD使用抗vWF与PEDF表达在对照子宫内膜呈负相关，但没有在卵巢子宫内膜异位症lesions.Conclusions：我们的研究结果表明，PEDF表达减少，MVD增加卵巢子宫内膜异位症的发病机制可能发挥重要作用。(C)2012爱思唯尔爱尔兰有限公司保留所有权利。
Objectives: To determine whether women with endometriosis have altered expression of pigment epithelium-derived factor (PEDF) in ovarian endometriotic lesions as compared to women without endometriosis.Study design: Ectopic and eutopic and normal endometrial tissues were sampled from 40 women with ovarian endometriosis and 20 control women, respectively. Endometrial PEDF expression and microvascular density (MVD) using an antibody to von Willebrand factor (vWF) and alpha-smooth muscle actin (alpha-SMA) were evaluated by using immunohistochemical staining.Results: We detected decreased PEDF expression and increased MVD using anti-vWF and -alpha-SMA in ovarian endometriotic lesions in women with endometriosis compared with the control group. In women with endometriosis, the MVD using anti-vWF and -alpha-SMA but not PEDF expression in ovarian endometriotic lesions correlated with the size of ovarian endometriotic cysts and the severity of the disease. Moreover, the MVD using anti-vWF was negatively correlated with PEDF expression in control endometrium but not in ovarian endometriotic lesions.Conclusions: Our results suggest that decreased PEDF expression and increased MVD in ovarian endometriotic lesions might play an important role in the pathogenesis of ovarian endometriosis. (C) 2012 Elsevier Ireland Ltd. All rights reserved.