Magnetite loaded Polypeptide-PLGA multifunctional microbubbles for dual-mode US/MR imaging
Magnetite loaded Polypeptide-PLGA multifunctional microbubbles for dual-mode US/MR imaging
复制标题
用于双模式 US/MR 成像的磁铁矿负载多肽-PLGA 多功能微泡。
DOI：
10.1002/cmmi.1675
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发表时间：
2016-03-01
影响因子：
--
通讯作者：
Duan, Yourong
中科院分区：
文献类型：
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作者：
Sun, Ying;Zhu, Yunkai;Duan, Yourong
采用改良的复乳法制备了具有超顺磁性的磁性聚肽-PLGA多功能微泡（Fe 3 O 4/Polypeptide-PLGA MMBs），并将其用于前列腺癌的超声/磁共振（US/MR）双模式成像。采用傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）、透射电镜（TEM）、扫描电镜（SEM）、原子吸收光谱（AAS）和振动样品磁强计（VSM）等测试手段对MMB的合成进行了表征。制备的MMBs直径约为700 nm，MTT法证实其安全性。普鲁士蓝染色显示靶向Fe 3 O 4/Polypeptide-PLGA MMB增强了细胞的摄取效率。在细胞粘附研究中，与阴性对照PC 3细胞相比，MMB对LNCaP细胞的粘附显著更高。体外实验结果表明，这些MMB可以增强前列腺癌的US和MR成像。
Magnetite loaded Polypeptide-PLGA multifunctional microbubbles (Fe3O4 /Polypeptide-PLGA MMBs) that show superparamagnetic properties were prepared by a modified double emulsion method and employed as imaging agent for dual-mode Ultrasound/Magnetic resonance (US/MR) imaging of prostatic cancer. The successful synthesis of MMBs was determined by Fourier Transform Infrared Spectrometer (FTIR), X-ray diffraction (XRD), Transmission Electron Microscope (TEM), Scanning Electron Microscope (SEM), Atomic Absorption Spectroscopy (AAS) and vibrating sample magnetometer (VSM). The as-prepared MMBs had a diameter of 700 nm and were quite safe as confirmed by MTT assays. Prussian Blue Staining showed that targeted Fe3O4 /Polypeptide-PLGA MMBs enhanced the cellular uptake efficiency. In cell attachment study, adherence of MMBs was significantly higher to LNCaP cells compared with negative control PC3 cells. The in vitro results demonstrated that these MMBs could enhance both US and MR imaging of prostatic cancer.