Genistein inhibits proliferation and in vitro invasive potential of human prostatic cancer cell lines.
Genistein inhibits proliferation and in vitro invasive potential of human prostatic cancer cell lines.
复制标题
金雀异黄素抑制人前列腺癌细胞系的增殖和体外侵袭潜力。
作者：
J. Santibañez;A. Navarro;Jorge Martínez
金雀异黄素 - 一种具有抗肿瘤活性的天然黄酮化合物 - 已被提议作为预防激素依赖性癌症转移能力表达的有效药物。本研究旨在评估金雀异黄素抑制具有不同侵袭潜力的肿瘤前列腺细胞的增殖和体外侵袭能力表达的功效。在细胞培养系统中，金雀异黄素似乎具有细胞毒性，并可通过 PC-3 细胞（所研究的更具侵袭性的侵袭性细胞系）的材料屏障抑制迁移。 DU-145 和 LNCaP 细胞比 PC-3 侵袭性更小，在增殖率以及 u-PA 和 72 kDa 明胶酶分泌抑制方面受金雀异黄素影响较小。对所研究的三种细胞系中酪氨酸磷蛋白水平的测量还表明，PC-3 细胞是最敏感的细胞，可能的分子靶点是 130 kDa 的膜结合蛋白。
Genistein -a natural flavone compound with antitumor activity- has been proposed as an effective agent to prevent the expression of metastasic capacity in hormone-dependent cancers. The present study represents an effort to assess the efficacy of Genistein in inhibiting the proliferation and expression of the in vitro invasive capacity of tumoral prostatic cells with different invasive potential. In a cell culture system, genistein appeared to be cytotoxic and inhibitory of miaration through a Material barrier to PC-3 cells, the more aggressive invasive cell-line studied. DU-145 and LNCaP cells, which are less invasive than PC-3, are less affected by Genistein both with respect to proliferation rate and inhibition of u-PA and 72 kDa Gelatinase secretion. Measurement of the level of tyrosine-phosphoproteins in the three cell lines studied also showed that PC-3 cells are the most sensitive cells, with a possible molecular target in a membrane-bound protein of 130 kDa.