Expression of heme oxygenase in the eutopic and ectopic endometrium in patients with adenomyosis
Expression of heme oxygenase in the eutopic and ectopic endometrium in patients with adenomyosis
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DOI：
10.3109/09513590.2012.683064
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发表时间：
2012-10
影响因子：
2
通讯作者：
Y. Iwahara;A. Nagai;N. Yoshiki;Kaori Igarashi;K. Yamashita;T. Kubota
中科院分区：
文献类型：
--
作者：
Y. Iwahara;A. Nagai;N. Yoshiki;Kaori Igarashi;K. Yamashita;T. Kubota
血红素加氧酶 (HO) 是催化血红素降解为铁、一氧化碳和胆绿素的限速酶。这种酶在细胞稳态中具有重要功能，包括氧化负荷、细胞凋亡和炎症的调节。 HO 的两种亚型，即诱导型 HO-1 和组成型 HO-2，在整个月经周期的正常人类子宫内膜中表达并发挥作用，但几乎没有证据表明 HO 在子宫腺肌病（即壁内异位子宫内膜组织的发生）中表达和行为。本研究的目的是调查患有子宫腺肌病的女性在月经周期的在位和异位子宫内膜中两种 HO 亚型的存在和定位。还评估了与 HO-1 表达相关的氧化应激和细胞凋亡。 HO-1和HO-2在在位和异位子宫内膜中均有表达，且异位子宫内膜中HO-1和HO-2的水平低于在位子宫内膜。 HO表达的周期性变异在月经周期期间在异位子宫内膜中消失，而这种变异在在位子宫内膜中是明显的。此外，HO-1 表达与在位子宫内膜中的细胞凋亡事件相对应。 HO-2 的组成型表达与子宫内膜增殖和降解相对应。这些结果表明 HO-1 和 HO-2 在子宫腺肌病的病理生理学中贡献很小。
Heme oxygenase (HO) is the rate-limiting enzyme that catalyzes the degradation of heme into iron, carbon monoxide, and biliverdin. This enzyme has important functions in cellular homeostasis, including the regulation of oxidative load, apoptosis, and inflammation. Two isoforms of HO, the inducible HO-1 and the constitutive HO-2, are expressed and are known to play a role in the normal human endometrium throughout the menstrual cycle, but there is little evidence for HO expression and behavior in adenomyosis, which is the occurrence of intramural ectopic endometrial tissue. The aim of this study was to investigate the presence and localization of the two HO isoforms in both eutopic and ectopic endometrium of women with adenomyosis during the menstrual cycle. The oxidative stress and apoptosis related to HO-1 expression were also assessed. The expression of HO-1 and HO-2 in both eutopic and ectopic endometrium was confirmed, and their levels in the ectopic endometrium were lower than those in the eutopic endometrium. The cyclic variability of HO expression was lost in the ectopic endometrium during the menstrual cycle, whereas this variability was apparent in the eutopic endometrium. Moreover, HO-1 expression corresponded to apoptotic events in the eutopic endometrium. Constitutive HO-2 expression corresponded to endometrial proliferation and degradation. These results reveal that both HO-1 and HO-2 contribute little in the pathophysiology of adenomyosis.