Paternal inheritance in mealybugs (Hemiptera: Coccoidea: Pseudococcidae)
Paternal inheritance in mealybugs (Hemiptera: Coccoidea: Pseudococcidae)
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DOI：
10.1007/s00114-014-1218-7
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发表时间：
2014-10-01
影响因子：
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通讯作者：
Ghanim, Murad
中科院分区：
文献类型：
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作者：
Kol-Maimon, Hofit;Mendel, Zvi;Ghanim, Murad
粉虱具有单倍二倍体繁殖系统，具有父本基因组消除（PGE）；雄性在受精后不久就变成二倍体，但在胚胎发生过程中，雄性父本染色体组变成异染色质（HC），因此不活跃。先前的研究表明，父系基因可以从雄性粉蚧传给它们的儿子，但不一定是任何儿子传给下一代。我们采用了两种粉蚧品种——榕树平球菌 (Signoret) 和柑橘平球菌 (Risso) 之间的杂交，以及信息素吸引方式不同的两种粉虱种群之间的杂交，以证明从雄性到 F2 到 F1 雄性杂种的父系遗传。通过三代监测两个性状：雄性信息素吸引模式（表型）和内部转录间隔区 2 (ITS2) 基因片段的序列（基因型）。我们的结果表明，粉虱的父系遗传可以从雄性通过 F1 雄性（父系）传给其 F2 后代。 F2 回交杂交雄性表达父本表型和 ITS2 基因型，尽管它们的母亲起源于母系群体。进一步的结果揭示了粉蚧遗传的其他迄今为止未知的方面，例如两个物种之间的杂交导致F1杂种雌性产生的F2杂种雌性中缺乏父系性状。此外，这两个物种之间的杂交提出了一个问题：未引诱的雄性是否在榕树和柑橘树之间的相互作用中发挥作用。
Mealybugs have a haplodiploid reproduction system, with paternal genome elimination (PGE); the males are diploid soon after fertilization, but during embryogenesis, the male paternal set of chromosomes becomes heterochromatic (HC) and therefore inactive. Previous studies have suggested that paternal genes can be passed on from mealybug males to their sons, but not necessarily by any son, to the next generation. We employed crosses between two mealybug species-Planococcus ficus (Signoret) and Planococcus citri (Risso)-and between two populations of P. ficus, which differ in their mode of pheromone attraction, in order to demonstrate paternal inheritance from males to F2 through F1 male hybrids. Two traits were monitored through three generations: mode of male pheromone attraction (pherotype) and sequences of the internal transcribed spacer 2 (ITS2) gene segment (genotype). Our results demonstrate that paternal inheritance in mealybugs can occur from males to their F2 offspring, through F1 males (paternal line). F2 backcrossed hybrid males expressed paternal pherotypes and ITS2 genotypes although their mother originated through a maternal population. Further results revealed other, hitherto unknown, aspects of inheritance in mealybugs, such as that hybridization between the two species caused absence of paternal traits in F2 hybrid females produced by F1 hybrid females. Furthermore, hybridization between the two species raised the question of whether unattracted males have any role in the interactions between P. ficus and P. citri.