A NOVEL IMMUNOASSAY FOR THE QUANTITATION OF HUMAN C4 GENE-PRODUCTS
A NOVEL IMMUNOASSAY FOR THE QUANTITATION OF HUMAN C4 GENE-PRODUCTS
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DOI：
10.1159/000463134
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发表时间：
1990-03-01
期刊：
影响因子：
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通讯作者：
HAMILTON, RG
中科院分区：
文献类型：
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作者：
MOULDS, JM;ARNETT, FC;HAMILTON, RG
利用C4A和C4B分子上携带的识别Rodgers 1和Chido 1表位的小鼠单克隆抗体和激活C1的热聚集IgG，建立了一种定量总C4以及C4A和C4B的免疫测定方法。试验间变异率分别为12.4%、11.5和10.8%。通过103个随机白色对照，将免疫分析法与放射免疫扩散法测定总C4进行比较，Pearson积差相关系数为0.81。三个基因总c4缺陷个体在所有三种检测中均无反应。该激活分析具有特异性、可重复性，优于现有的C4A和C4B定量和杂合C4零态检测方法。
Utilizing mouse monoclonal antibodies which recognize Rodgers 1 and Chido 1 epitopes carried on the C4A and C4B molecules, and heat-aggregated IgG to activate C1, an immunoassay was developed for the quantitation of total C4 as well as C4A and C4B. Interassay variation was 12.4, 11.5 and 10.8%, respectively. The immunoassay was compared to the quantitation of total C4 by radial immunodiffusion by testing 103 random white controls and gave a Pearson''s product-moment correlation coefficient of 0.81. Three genetic total-C4-deficient individuals were nonreactive in all three assays. This activated assay is specific, reproducible, and superior to existing methods for the quantitation of C4A and C4B and detection of the heterozygous C4 null state.