Improving enzymatic hydrolysis efficiency of corncob residue through sodium sulfite pretreatment
Improving enzymatic hydrolysis efficiency of corncob residue through sodium sulfite pretreatment
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亚硫酸钠预处理提高玉米芯渣酶解效率
DOI：
10.1007/s00253-019-10050-7
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发表时间：
2019-09-01
影响因子：
5
通讯作者：
Wang, Haisong
中科院分区：
文献类型：
--
作者：
Chen, Hang;Jiang, Lifeng;Wang, Haisong
研究了亚硫酸钠预处理对玉米芯渣脱木素率、纤维素含量、酶解效率和葡萄糖产率的影响。最佳预处理条件为：亚硫酸钠用量12%，pH值7，温度160 °C，保温时间20 min。在此条件下，预处理后残渣中纤维素含量为85.17%，废液中木质素磺酸钠的磺化度为0.677 mmol/g。预处理后的脱木素率也达到77.45%。以纤维素酶（5 FPU/g底物）和β-葡萄糖苷酶（10 IU/g底物）对预处理后的CCR进行酶解48 h。用纤维素酶（20 FPU/g底物）和β-葡萄糖苷酶（10 IU/g底物）水解未处理的CCR 48 h。结果表明，亚硫酸钠预处理提高了酶解效率和葡萄糖得率，分别提高了28.80%和20.10%。这些结果表明，即使纤维素酶用量较低，也能生产出较高酶解效率的底物，并从亚硫酸钠预处理的CCR中获得可用于油田和混凝土添加剂的木质素磺酸钠。
The effects of sodium sulfite pretreatment on the delignification rate, cellulose content, enzymatic hydrolysis efficiency, and glucose yield of corncob residues (CCR) were investigated. The optimum pretreatment conditions were as follows: 12% sodium sulfite, with a pH value of 7, a temperature of 160 °C, and a holding time of 20 min. Under the optimal conditions, the cellulose content in the pretreated residue was 85.17%, and sodium lignosulfonate with a sulfonation degree of 0.677 mmol/g was obtained in the waste liquids. A delignification rate of 77.45% was also achieved after the pretreatment. Enzymatic hydrolysis of pretreated CCR was carried out with cellulase (5 FPU/g substrate) and β-glucosidase (10 IU/g substrate) for 48 h. The untreated CCR were hydrolyzed using cellulase (20 FPU/g substrate) and β-glucosidase (10 IU/g substrate) for 48 h. The comparison results showed that sodium sulfite pretreatment improved the enzymatic hydrolysis efficiency and glucose yield, which increased by 28.80% and 20.10%, respectively. These results indicated that despite the application of low cellulase dosage, high enzymatic hydrolysis efficiency substrate could be produced, and the sodium lignosulfonate which can be used for oilfields and concrete additives was obtained from the sodium sulfite–pretreated CCR.