Defective acute inflammation in Crohn's disease: a clinical investigation
Defective acute inflammation in Crohn's disease: a clinical investigation
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DOI：
10.1016/s0140-6736(06)68265-2
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发表时间：
2006-02-25
期刊：
影响因子：
168.9
通讯作者：
Segal, AW
中科院分区：
文献类型：
--
作者：
Marks, DJB;Harbord, MWN;Segal, AW
背景 克罗恩病的病因尚未得到机械证实。我们检验了该疾病是一种由先天免疫受损引起的免疫缺陷的假设。方法我们通过量化急性创伤后中性粒细胞的募集和细胞因子的产生、暴露于炎症介质后培养的单核细胞衍生的巨噬细胞的白介素 8 分泌以及响应于局部炎症和血管的变化来研究患者和对照的炎症反应。 皮下注射热灭活的大肠杆菌。结果在克罗恩病患者中，直肠、回肠或皮肤创伤导致中性粒细胞积聚异常低（与健康个体的差异为 79%，n=8，p=0.0003；57%，n=3，p=0.05；50%，n=13，P
Background The cause of Crohn's disease has not been mechanistically proven. We tested the hypothesis that the disease is a form of immunodeficiency caused by impaired innate immunity.Methods We investigated inflammatory responses in patients and controls by quantifying neutrophil recruitment and cytokine production after acute trauma, interleukin 8 secretion by cultured monocyte-derived macrophages after exposure to inflammatory mediators, and local inflammatory and vascular changes in response to subcutaneous injection of heat-killed Escherichia coli.Findings In patients with Crohn's disease, trauma to rectum, ileum, or skin led to abnormally low neutrophil accumulation (differences from healthy individuals of 79%, n=8, p=0.0003; 57%, n=3, p=0.05; 50%, n=13, P