Effect of methyl jasmonate on carotenoids biosynthesis in germinated maize kernels
Effect of methyl jasmonate on carotenoids biosynthesis in germinated maize kernels
复制标题
茉莉酸甲酯对发芽玉米籽粒类胡萝卜素生物合成的影响
DOI：
10.1016/j.foodchem.2019.125525
复制
发表时间：
2020-03-01
期刊：
影响因子：
8.8
通讯作者：
Liu, Chunquan
中科院分区：
文献类型：
--
作者：
Luo, Hao;He, Weiwei;Liu, Chunquan
研究了不同浓度茉莉酸甲酯（MeJA）对萌发玉米籽粒类胡萝卜素积累、自由基清除活性和脯氨酸含量的影响。MeJA处理促进了类胡萝卜素积累、自由基清除活性和脯氨酸积累，而水杨基异羟肟酸（SHAM）则降低了类胡萝卜素积累。当用0.5 μ M MeJA处理时，叶黄素含量显著增加42.5%。此外，7个胡萝卜素基因的转录表达进行了探索MeJA和SHAM。结果表明，0.5 μ M MeJA显著提高了PSY、PDS、ZDS、LCYB、LCYE、BCH 1、CYP97C等基因的表达水平及其转录水平，这些基因的表达水平与类胡萝卜素含量密切相关。MeJA处理影响类胡萝卜素合成基因，导致类胡萝卜素积累。这些新的发现将有助于开发创新的方法来富集发芽玉米籽粒中的叶黄素，以进一步开发功能性食品材料。
This study investigated effects of different concentrations of methyl jasmonate (MeJA) on carotenoids accumulation, radical scavenging activity and proline content in germinated maize kernels. MeJA treatment promoted carotenoids accumulation, radical scavenging activity and proline accumulation, while salicyl hydroxamic acid (SHAM) reduced carotenoids accumulation. There was a significant increase of 42.5% in lutein content when treated with 0.5 mu M MeJA. Furthermore, the transcriptional expression of seven carotenogenic genes were explored by MeJA and SHAM. The results showed that 0.5 mu M MeJA significantly increased the gene expression levels of PSY, PDS, ZDS, LCYB, LCYE, BCH1, CYP97C, and their transcript levels, which were strongly associated with carotenoids content. Treatment of MeJA affected the carotenoids biosynthesis gene and led to the accumulation of carotenoids. These new findings would help to develop innovative approach for enrichment of lutein in germinated maize kernels for further development of functional food materials.