Phenethyl Isothiocyanate Induces Apoptosis Through ROS Generation and Caspase-3 Activation in Cervical Cancer Cells.
Phenethyl Isothiocyanate Induces Apoptosis Through ROS Generation and Caspase-3 Activation in Cervical Cancer Cells.
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DOI：
10.3389/fphar.2021.673103
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发表时间：
2021
影响因子：
5.6
通讯作者：
Islam N
中科院分区：
文献类型：
--
作者：
Shoaib S;Tufail S;Sherwani MA;Yusuf N;Islam N
最新研究表明，由于宫颈癌细胞产生耐药性，目前的化疗药物效果不佳，因此其使用范围有限。目前研究人员主要关心的是发现安全有效的抗增殖植物化学物质，这些化学物质可以帮助抗击宫颈癌。以前的研究表明，从十字花科植物中获得的苯乙基异硫氰酸酯可能对许多癌症具有抗癌潜力，它针对不同的信号通路发挥化学预防和治疗作用。这为研究异硫氰酸苯乙酯对宫颈癌的治疗潜力提供了依据。在本研究中，不同剂量的异硫氰酸苯乙酯单独和与顺铂联合治疗宫颈癌细胞。异硫氰酸苯乙酯单独作用足以引起宫颈癌细胞的核固缩和碎裂，并诱导细胞凋亡，但与顺铂联合应用时有明显的协同作用。此外，异硫氰酸苯乙酯处理以剂量依赖的方式增加了宫颈癌细胞内ROS的产生。此外，对异硫氰酸苯乙酯诱导线粒体产生活性氧和激活caspase的研究表明，异硫氰酸苯乙酯显著激活caspase-3。
The latest research shows that current chemotherapeutics are ineffective because of the development of resistance in cervical cancer cells, and hence, their scope of use is limited. The main concern of researchers at the moment is the discovery of safe and effective antiproliferative plant chemicals that can aid in the battle against cervical cancer. Previous studies have shown the possible anticancer potential of phenethyl isothiocyanate obtained from cruciferous plants for many cancers, which targets various signaling pathways to exercise chemopreventive and therapeutic effects. This provides the basis for studying phenethyl isothiocyanate's therapeutic potential against cervical cancer. In the present study, cervical cancer cells were treated with various doses of phenethyl isothiocyanate, alone and in combination with cisplatin. Phenethyl isothiocyanate alone was sufficient to cause nucleus condensation and fragmentation and induce apoptosis in cervical cancer cells, but evident synergistic effects were observed in combination with cisplatin. In addition, phenethyl isothiocyanate treatment increased the production of intracellular ROS in a dose-dependent manner in cervical cancer cells. Furthermore, investigation of phenethyl isothiocyanate induced mitochondrial reactive oxygen species production, and activation of caspases showed that phenethyl isothiocyanate significantly activated caspase-3.