Dried blood spots are an acceptable and useful HIV surveillance tool in a remote developing world setting
Dried blood spots are an acceptable and useful HIV surveillance tool in a remote developing world setting
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DOI：
10.1258/0956462041944402
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发表时间：
2004-10-01
影响因子：
1.4
通讯作者：
Solomon, S
中科院分区：
文献类型：
--
作者：
Solomon, SS;Pulimi, S;Solomon, S
滤纸上干血斑（DBS）的酶联免疫吸附试验和Western blot分析已被证明与血清分析一样敏感和特异性，因此可能是偏远地区具有成本效益和文化适宜的艾滋病毒血清流行率检测工具。本研究考察了印度安得拉邦一家门诊诊所热带农村人口对DBS的接受程度，该诊所在2001年3月至4月期间向每五名寻求普通医疗服务的患者提供了参与DBS的机会。所有参与研究的1413名患者都同意参与并提供DBS检查。该样本的总体HIV血清阳性率为2.8%。在参与者中，51.7%为男性，93.2%年龄在18至40岁之间，85.3%已婚，29.7%有工作，47.6%没有受过教育，73.1%居住在农村。在单变量分析中，生殖器疣史（P = 0.01）、性传播疾病史（P = 0.001）、婚前性行为史（P = 0.002）、与商业性工作者发生性接触史（P = 0.003）、被雇佣史（P = 0.011）以及为医疗目的注射10次以上（P = 0.006）均与感染艾滋病毒相关。鉴于这些诊所参与者都有接受检测的一致意愿，我们得出结论，DBS是一种有用的、具有成本效益的工具，可用于农村热带地区的艾滋病毒服务监测。
Enzyme-linked immunosorbent assay and Western blot analysis of dried blood spots (DBS) on filter paper have been shown to be as sensitive and specific as analysis of serum, and therefore may be a cost-effective and culturally appropriate HIV seroprevalence tool in remote areas. This study examines the acceptability of DBS in a tropical, rural population from an outpatient clinic in Andhra Pradesh, India, where participation was offered to every fifth patient seeking general medical care between March and April 2001. All 1413 patients approached for the study agreed to participate and provide a DBS for examination. The overall HIV seroprevalence in this sample was 2.8%. Of the participants, 51.7% were male, 93.2% were between the ages of 18 and 40, 85.3% were married, 29.7% were employed, 47.6% had no education and 73.1%, resided in a rural setting. In the univariate analysis, history of genital warts (P = 0.01), sexually transmitted disease (P = 0.001), premarital sexual intercourse (P = 0.002), sexual contact with a commercial sex worker (P = 0.003), being employed (P = 0.011) and having more than 10 injections for medical purposes (P = 0.006) all correlated with being HIV-infected.Given the uniform willingness of these clinic attendees to be tested, we conclude that DBS is a useful, cost-effective tool in HIV serosurveillance in a rural, tropical setting.