Comparisons of microRNA expression profiles in vitreous humor between eyes with macular hole and eyes with proliferative diabetic retinopathy
Comparisons of microRNA expression profiles in vitreous humor between eyes with macular hole and eyes with proliferative diabetic retinopathy
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DOI：
10.1007/s00417-014-2692-5
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发表时间：
2015-03-01
影响因子：
2.7
通讯作者：
Hirakata, Akito
中科院分区：
文献类型：
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作者：
Hirota, Kazunari;Keino, Hiroshi;Hirakata, Akito
MicroRNAs(MiRNAs)是一种小的非编码RNA，调节靶mRNAs的活性。我们比较了视网膜增殖性糖尿病视网膜病变(PDR)和黄斑裂孔(MH)患者玻璃体中miRNAs的表达情况。我们用microRNA-PCR Panel方法检测了168例玻璃体和血清标本中的miRNAs，MH组玻璃体表达的miRNAs平均为63(55-69)个，PDR组为86(65-117)个。MH患者血清中miRNAs平均表达162(159-167)个，PDR患者为142(115-160)个。26个miRNAs在MH和PDR眼的玻璃体中均有表达。尽管26个miRNAs中的20个(73%)在MH和PDR眼中的表达水平没有显著差异，但26个miRNAs中有6个(24%)(hsa-miR-15a、hsa-miR320a、hsa-miR-320b、hsa-miR-93、hsa-miR-29a和hsa-miR-423-5p)在PDR眼中的表达水平显著升高。此外，PDR组玻璃体-血清中hsa-miR-23a、hsa-miR-320a和hsa-miR-320b三种miRNAs的平均倍数变化均显著高于MH组，与血管生成和纤维化相关的几种miRNAs在PDR眼玻璃体中的表达显著升高。需要进一步的研究来了解miRNAs在眼睛的生物功能中所起的作用。
MicroRNAs (miRNAs) are small noncoding RNAs which regulate the activities of target mRNAs. We compared the expression profiles of the miRNAs in the vitreous of eyes with macular hole (MH) to that in eyes with proliferative diabetic retinopathy (PDR).Vitreous and whole blood samples were collected from four patients with MH and from four patients with PDR. We assayed for 168 miRNAs in the vitreous and serum samples by the microRNA PCR Panel method.The mean number of miRNAs expressed in the vitreous was 63 (55-69) in eyes with MH and 86 (65-117) in eyes with PDR. The mean number of miRNAs expressed in the serum was 162 (159-167) in the MH patients and 142 (115-160) in the PDR patients. Twenty-six miRNAs were expressed in the vitreous of both MH and PDR eyes. Although there was no significant difference in the levels of 20 of the 26 (73 %) miRNAs expressed in both MH and PDR eyes, six of 26 miRNAs (24 %) (hsa-miR-15a, hsa-miR320a, hsa-miR-320b, hsa-miR-93, hsa-miR-29a, and hsa-miR-423-5p) were expressed significantly more highly in PDR eyes. In addition, the mean fold changes of three miRNAs, hsa-miR-23a, hsa-miR-320a, and hsa-miR-320b, in the vitreous to serum were significantly higher in the PDR group than in the MH group.The expression of several miRNAs related to angiogenesis and fibrosis was expressed significantly higher in the vitreous of eyes with PDR. Further studies are needed to understand the role played by the miRNAs in the biological function of the eye.