EFFECT OF CYCLIC RGD PEPTIDE ON CELL-ADHESION AND TUMOR-METASTASIS
EFFECT OF CYCLIC RGD PEPTIDE ON CELL-ADHESION AND TUMOR-METASTASIS
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DOI：
10.1016/0006-291x(91)91950-h
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发表时间：
1991-05-31
影响因子：
3.1
通讯作者：
OHBA, M
中科院分区：
文献类型：
--
作者：
KUMAGAI, H;TAJIMA, M;OHBA, M
采用液相法合成了几种含有L-精氨酸-甘氨酸-L-天冬氨酸-RGD序列的环肽，并研究了它们对小鼠B16黑色素瘤细胞在纤连蛋白包被的微孔上粘附的影响。Cyclo（GRGDSPA）在比线性形式低20倍的浓度下抑制细胞附着。合成肽对细胞粘附有抑制作用，其活性顺序为：cyclo（GRGDSPA）> cyclo（GRGDS）> cyclo（GRGDSP）> cyclo（GRGDS）> cyclo（RGDSP），cyclo（RGDSPA）。Cyclo（GRGDSPA）在抑制细胞与玻连蛋白的附着方面比在与纤连蛋白的竞争附着方面更有效，如在GRGDSP的情况中所报道的。
Several kinds of cyclic peptides containing an L-arginine-glycine-L-aspartic acid RGD sequence were synthesized by the liquid phase method, and we investigated their effects on the attachment of mouse B16 melanoma cells onto fibronectin-coated well. Cyclo(GRGDSPA) inhibited the cell attachment at a 20-fold lower concentration than the linear form. The cell adhesion was inhibited by the synthetic peptides with the following relative order of activity: cyclo(GRGDSPA) ⪢ cyclo(GRGD) > cyclo(RGDS), cyclo(GRGDSP) > cyclo(GRGDS) > cyclo(RGDSP), cyclo(RGDSPA). Cyclo(GRGDSPA) was more effective at inhibiting cell attachment to vitronectin than it was at competing with fibronectin attachment, as reported in the case of GRGDSP.Moreover, cyclo(GRGDSPA) significantly reduced the formation of colonies in mice injected with B16-FE7 melanoma cells.