Patterns of Corpora Lutea Growth and Progesterone Secretion in Sows with Thrifty Genotype and Leptin Resistance due to Leptin Receptor Gene Polymorphisms (Iberian Pig)
Patterns of Corpora Lutea Growth and Progesterone Secretion in Sows with Thrifty Genotype and Leptin Resistance due to Leptin Receptor Gene Polymorphisms (Iberian Pig)
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DOI：
10.1111/j.1439-0531.2011.01776.x
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发表时间：
2011-12-01
影响因子：
1.7
通讯作者：
Gonzalez-Bulnes, A.
中科院分区：
文献类型：
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作者：
Gonzalez-Anover, P.;Encinas, T.;Gonzalez-Bulnes, A.
目前的研究旨在比较 10 头肥胖/瘦素抗性基因型母猪（伊比利亚猪）和 10 头瘦肉商业杂交母猪（大白猪 x 长白猪）的黄体功能（通过黄体动力学和黄体酮分泌进行测量）。在所有动物中，发情周期与孕激素同步，并由外源性促性腺激素诱导排卵。此后，在发情检测时测定卵泡的数量和大小以及血浆雌二醇浓度，并从周期的第4天至第12天评估黄体的数量和大小以及孕酮浓度。卵泡群体基因型、雌二醇浓度和排卵率之间没有差异（伊比利亚母猪为 15.2 +/- 1.3，LWxL 母猪为 12.7 +/- 1.8）；然而，伊比利亚母猪出现黄体囊肿的比例高于对照母猪（分别为 66.7% 和 30%；p < 0.05）。在这两个品种中，黄体总面积和血浆孕酮浓度从第 4 天到第 8 天呈线性增长 (p < 0.01)，并在第 8 天到第 12 天之间保持更加稳定，基因型之间没有显着差异。总之，目前的研究支持排卵过程和黄体功能并不是瘦素抵抗和肥胖猪模型繁殖力的主要限制因素。
The current study aimed to compare luteal function, as measured by corpora lutea dynamics and progesterone secretion, in 10 sows with obesity/leptin resistance genotype (Iberian pig) and 10 females of lean commercial crosses (Large White x Landrace). In all the animals, the oestrous cycle was synchronized with progestagens, and ovulation was induced by exogenous gonadotrophins. Thereafter, number and size of follicles and plasma oestradiol concentration were determined at oestrus detection, and number and size of corpora lutea and progesterone concentration were evaluated from Day 4 to 12 of the cycle. There were no differences between genotypes in follicle population and oestradiol concentration, and ovulation rate (15.2 +/- 1.3 in Iberian vs 12.7 +/- 1.8 in LWxL sows); however, there was a higher percentage of Iberian than control sows showing luteal cysts (66.7% vs 30%, respectively; p < 0.05). In both breeds, both total luteal area and plasma progesterone concentration grew linearly from Day 4 to 8 (p < 0.01) and remained more stable between Days 8 and 12, without significant differences between genotypes. In conclusion, current study supports that ovulatory processes and luteal functionality are not the main limiting factors for prolificacy in a pig model of leptin resistance and obesity.