Catecholamines and sympathomimetic drugs decrease early Salmonella Typhimurium uptake into porcine Peyer's patches
Catecholamines and sympathomimetic drugs decrease early Salmonella Typhimurium uptake into porcine Peyer's patches
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DOI：
10.1111/j.1574-695x.2007.00348.x
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发表时间：
2008-01-01
影响因子：
--
通讯作者：
Price, Lisa D.
中科院分区：
文献类型：
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作者：
Brown, David R.;Price, Lisa D.
小肠的派伊尔集合淋巴结作为粘膜免疫的诱导位点以及包括沙门氏菌在内的侵入性肠道病原体的靶点。因为它们受含儿茶酚胺的肠神经支配，所以测试了内源性儿茶酚胺多巴胺和去甲肾上腺素或拟交感神经药物改变鼠伤寒沙门氏菌摄取到派尔集合淋巴结的假设。将猪空肠Peyer集合淋巴结外植体固定在Ussing室中，并接种鼠伤寒沙门氏菌DT 104的猪田间分离株。沙门氏菌从庆大霉素处理的组织中的回收率在细菌暴露于粘膜表面30至90分钟之间显著增加。添加神经传导阻滞剂石房蛤毒素（0.1 μ mol L-1）或多巴胺（30 μ mol L-1）的contraluminal方面的外植体减少细菌恢复后60分钟的沙门氏菌暴露。多巴胺的影响被模仿可卡因和甲基苯丙胺（30 μ mol L-1），其作用于儿茶酚胺能神经末梢，以增加突触神经递质的浓度。这些结果表明，肠道儿茶酚胺能神经调节沙门氏菌定植的派尔氏集结在感染的最早阶段，部分通过改变上皮吸收的细菌。
Peyer's patches of the small intestine serve as inductive sites for mucosal immunity as well as targets for invasive enteropathogens, including Salmonella. Because they are innervated by catecholamine-containing enteric nerves, the hypothesis that the endogenous catecholamines dopamine and norepinephrine or sympathomimetic drugs alter Salmonella Typhimurium uptake into Peyer's patches was tested. Porcine jejunal Peyer's patch explants were mounted in Ussing chambers and inoculated with a porcine field isolate of Salmonella Typhimurium DT104. Salmonella recovery from gentamicin-treated tissues increased significantly between 30 and 90 min of bacterial exposure to the mucosal surface. Addition of the neuronal conduction blocker saxitoxin (0.1 mu mol L-1) or dopamine (30 mu mol L-1) to the contraluminal aspect of explants decreased bacterial recovery after 60 min of Salmonella exposure. The effects of dopamine were mimicked by cocaine and methamphetamine (30 mu mol L-1), which act on catecholaminergic nerve terminals to increase synaptic neurotransmitter concentrations. These results suggest that enteric catecholaminergic nerves modulate Salmonella colonization of Peyer's patches at the earliest stages of infection, in part by altering epithelial uptake of bacteria.