Xenografting of gonadal tissues into mice as a possible method for conservation and utilization of porcine genetic resources
Xenografting of gonadal tissues into mice as a possible method for conservation and utilization of porcine genetic resources
复制标题
将性腺组织异种移植到小鼠体内作为保护和利用猪遗传资源的一种可能方法
DOI：
10.1111/j.1740-0929.2011.00919.x
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发表时间：
2011
期刊：
影响因子：
2
通讯作者：
Kaneko H
中科院分区：
文献类型：
--
作者：
Kikuchi K;Nakai M;Kashiwazaki N;Kaneko H
胚胎的体外生产，包括卵母细胞的体外成熟、受精和随后的胚胎培养，已成为研究猪配子发生和胚胎发生的最流行的方法。这些程序不仅可用于基础研究，而且使我们能够产生可存活的胚胎和后代，作为保护遗传资源和稀有动物品种的一种手段。最近，更先进的技术，如异种移植的性腺（睾丸和卵巢）组织到免疫缺陷的实验动物已经开发出来。与体外胚胎生产技术相结合，这种方法可以提供许多好处。我们一直在进行研究，以获得有关该方法应用于猪种的基本信息，并改进现有技术。最近，我们从异种移植的卵巢组织中获得了卵母细胞，并在裸鼠中生长，这些卵巢组织具有受精能力和发育成早期胚胎的能力。我们还从异种移植的睾丸组织中获得精子，并将其胞浆内注射到体外成熟的卵母细胞中以产生仔猪。在这里，我们讨论了进一步的可能性，保护和利用猪性腺组织异种移植到免疫缺陷小鼠。
In vitroproduction of embryos, includingin vitromaturation, fertilization of oocytes and their subsequent culture to the embryo stage, has become the most popular method of studying gametogenesis and embryogenesis in pigs. As well as their utility for basic studies, these procedures now enable us to generate viable embryos and offspring as a means of conserving genetic resources and rare animal breeds. Recently, more advanced technologies such as xenografting of gonadal (testicular and ovarian) tissues into immunodeficient experimental animals have been developed. In combination within vitroembryo production techniques, this approach may provide many benefits. We have been carrying out studies to acquire basic information about the application of this method to porcine species, and to improve the existing techniques. Recently, we obtained oocytes from ovarian tissue xenografted and grown in nude mice that had the capacity to be fertilized and the ability to develop into early‐stage embryos. We also obtained spermatozoa from the xenografted testicular tissues and injected them intracytoplasmically intoin vitro‐matured oocytes to produce piglets. Here we discuss the further possibilities of conservation and utilization of porcine gonadal tissue by xenografting into immunodeficient mice.