Effect of Simultaneous Exposure to Methanol and Toluene Vapor on Their Metabolites in Rats
Effect of Simultaneous Exposure to Methanol and Toluene Vapor on Their Metabolites in Rats
复制标题
同时暴露于甲醇和甲苯蒸气对其代谢物的影响
DOI：
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
I. Tanaka
中科院分区：
文献类型：
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作者：
H. Hori;Torn Ishidao;T. Oyabu;H. Yamato;Y. Morimoto;I. Tanaka
甲醇和甲苯蒸气同时暴露对大鼠代谢产物的影响日本职业与环境卫生大学健康科学学院环境管理I系-Wistar雄性大鼠分别和同时吸入甲醇和甲苯蒸气。分别于染毒6h后0、1.5、3h从尾部采血，用顶空气相色谱质谱(GC/MS)法测定甲醇和/或甲苯浓度。分别于暴露结束后0、18和42h检测尿中甲醇和甲苯的主要代谢产物甲酸和马尿酸。同时接触组的血甲苯浓度低于甲苯接触组。甲苯在血液中的生物半衰期同时暴露组明显短于单次暴露组。当甲醇和甲苯浓度均为300ppm时，同时接触组尿马尿酸排泄率明显低于甲苯接触组。这些结果表明，甲醇提高了甲苯的代谢速率。同时暴露组的血中甲醇浓度低于甲醇暴露组，但血液中甲醇的生物半衰期和尿甲酸浓度在单次暴露组和同时暴露组之间无显著差异。
Effect of Simultaneous Exposure to Methanol and Toluene Vapor on Their Metabolites in Rats: Hajime Horn, et al. Department of Environmental Management I, School of Health Sciences, University of Occupational and Environmental Health, Japan—Wistar male rats were exposed to methanol and toluene vapors by inhalation individually and simultaneously. Blood was sampled from the tail of the rats at 0, 1.5 and 3 h after a 6‐h exposure and the methanol and/or toluene concentrations were measured by the headspace‐gas chromatography‐mass spectrometry (GC/MS). Major metabolites of methanol and toluene, that is, formic acid and hippuric acid in urine were measured at 0,18 and 42 h after the end of the exposure. The toluene concentration in blood in the simultaneous exposure group was lower than that in the toluene exposure one. The biological half time of toluene in blood in the simultaneous exposure group was shorter than that in the single exposure one. The excretion rate of hippuric acid in urine in the simultaneous exposure group was significantly lower than that in the toluene exposure one when both the methanol and toluene concentrations were 300 ppm. These results suggest that the metabolic rate of toluene was enhanced by methanol. On the other hand, the biological half time of methanol in blood and the formic acid concentration in urine were not significantly different in the single and simultaneous exposure groups, although the methanol concentration in blood in the simultaneous exposure group was lower than that in the methanol exposure one.