Sorting by interfacial tension (SIFT): label-free selection of live cells based on single-cell metabolism
Sorting by interfacial tension (SIFT): label-free selection of live cells based on single-cell metabolism
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DOI：
10.1039/c8lc01328d
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发表时间：
2019-04-21
期刊：
影响因子：
6.1
通讯作者：
Abbyad, Paul
中科院分区：
文献类型：
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作者：
Pan, Ching W.;Horvath, Daniel G.;Abbyad, Paul
从群体中选择活细胞在许多生物学研究和生物技术中至关重要。我们在这里提出了一种新的液滴微流控方法，允许使用单个细胞的糖酵解活性对活细胞进行无标记和被动选择。据观察，使用一种特定的表面活性剂用于稳定液滴的形成，液滴的界面张力是非常敏感的pH值。孵育后，细胞乳酸盐释放的结果在液滴含有活细胞，以达到比其他液滴更低的pH值。这使得当受限液滴流过相对于流动方向对角定向的微制造沟槽时，能够分选含有活细胞的液滴。用人U87胶质母细胞瘤细胞证明了该技术用于仅选择含有活细胞的液滴，同时排除空液滴或含有死细胞的液滴。这种无标记的分选方法，被称为界面张力分选（SIFT），提出了一种基于代谢活动分选不同细胞类型的新策略。
Selection of live cells from a population is critical in many biological studies and biotechnologies. We present here a novel droplet microfluidic approach that allows for label-free and passive selection of live cells using the glycolytic activity of individual cells. It was observed that with the use of a specific surfactant utilized to stabilize droplet formation, the interfacial tension of droplets was very sensitive to pH. After incubation, cellular lactate release results in droplets containing a live cell to attain a lower pH than other droplets. This enables the sorting of droplets containing live cells when confined droplets flow over a microfabricated trench oriented diagonally with respect to the direction of flow. The technique is demonstrated with human U87 glioblastoma cells for the selection of only droplets containing a live cell while excluding either empty droplets or droplets containing a dead cell. This label-free sorting method, dubbed sorting by interfacial tension (SIFT) presents a new strategy to sort diverse cell types based on metabolic activity.