In Arabidopsis thaliana distinct alleles encoding mitochondrial RNA PROCESSING FACTOR 4 support the generation of additional 5′ termini of ccmB transcripts
In Arabidopsis thaliana distinct alleles encoding mitochondrial RNA PROCESSING FACTOR 4 support the generation of additional 5′ termini of ccmB transcripts
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DOI：
10.1007/s11103-017-0591-y
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发表时间：
2017-02
影响因子：
5.1
通讯作者：
Katrin Stoll;Christian Jonietz;S. Schleicher;C. C. des Francs-Small-C.;I. Small;S. Binder
中科院分区：
文献类型：
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作者：
Katrin Stoll;Christian Jonietz;S. Schleicher;C. C. des Francs-Small-C.;I. Small;S. Binder
在植物线粒体中，许多转录物的5′端是转录后产生的。我们发现五肽重复序列（PPR）蛋白RNA加工因子4（RPF 4）支持Landsbergerecta（Ler）和许多其他拟南芥生态型中ccmB转录本额外5′端的产生。RPF 4在Ler中的鉴定是应用一种正向遗传学方法，该方法得到了生态类型哥伦比亚（Col）互补研究的支持，该研究仅在从Ler引入RPF 4等位基因后才产生Ler型extraccmB 5 ′末端。对来自Lerand Col的RPF 4蛋白的不同部分组成的嵌合RPF 4蛋白的研究确定了N-末端和中央PPR基序的差异，这些差异解释了ecotype-specific变异incmB加工。这些结果与基于PPR基序识别核苷酸碱基的已知决定因素的inccmB转录本结合位点预测吻合得很好。
In plant mitochondria, the 5′ ends of many transcripts are generated post-transcriptionally. We show that the pentatricopeptide repeat (PPR) protein RNA PROCESSING FACTOR 4 (RPF4) supports the generation of extra 5′ ends ofccmBtranscripts in Landsbergerecta(Ler) and a number of otherArabidopsis thalianaecotypes. RPF4 was identified in Lerapplying a forward genetic approach supported by complementation studies of ecotype Columbia (Col), which generates the Ler-type extraccmB5′ termini only after the introduction of theRPF4allele from Ler. Studies with chimeric RPF4 proteins composed of various parts of the RPF4 proteins from Lerand Col identified differences in the N-terminal and central PPR motifs that explain ecotype-specific variations inccmBprocessing. These results fit well with binding site predictions inccmBtranscripts based on the known determinants of nucleotide base recognition by PPR motifs.