[N-Methyl-11C]Choline by on-columnreaction: a study on [^<11>C]CH_3I incorporation and the residual amount of precursor in the product
[N-Methyl-11C]Choline by on-columnreaction: a study on [^<11>C]CH_3I incorporation and the residual amount of precursor in the product
复制标题
柱上反应法测定[N-甲基-11C]胆碱：[^11C]CH_3I 掺入及产物中前体残留量的研究
DOI：
10.1002/jlcr.1838
复制
发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Pascali C.
中科院分区：
文献类型：
--
作者：
Bogni A.;Crispu O.;Fugazza L.;Cucchi C.;Laera L.;Iwata R.;CrippaF.;Bombardieri E.;Pascali C.
[N-甲基-11 C]胆碱是在室温下通过[11 C] CH 3 I与2-二甲氨基乙醇（DMAE）反应合成的，后者直接装载在弱阳离子交换柱上。大部分的努力都是为了减少产品最终溶液中残留前体的量，以使这种示踪剂更适合于大脑研究。在这个过程中，放射化学产率和残留的DMAE已被放置在与前体的起始量和所使用的冲洗条件，并与更“传统”的前体装载在C18盒或环路。最方便的分析技术和定量分析的条件，特别强调的前体的意见和指示，也报告。在我们认为是一个公平的妥协，[11 C] CH 3 I掺入产生约。起始物料残留量在8 - 12 ppm范围内时，可轻松达到90%。版权所有© 2010约翰威利父子有限公司.
[N‐Methyl‐11C]choline has been synthesized at room temperature by the reaction of [11C]CH3I with 2‐dimethylaminoethanol (DMAE), with the latter directly loaded on a weak cation‐exchange cartridge. Most of the efforts have been directed to reduce the amount of residual precursor in the product's final solution in order to make this tracer more suitable to brain studies. In the process, radiochemical yields and residual DMAE have been placed in relation with both the starting amount of precursor and the rinsing conditions used and compared with the more ‘traditional’ loading of the precursor on either a C18 cartridge or a loop. Comments and indications on the most convenient analytical technique and conditions for quantitative analysis, with particular emphasis on the precursor, are also reported. Under what we believe to be a fair compromise, [11C]CH3I incorporation yields of ca. 90% were easily achieved with a residual amount of starting material in the 8‐ to 12‐ppm range. Copyright © 2010 John Wiley & Sons, Ltd.