PROTEINS ENCODED BY AN AUXIN-REGULATED GENE FAMILY OF TOBACCO SHARE LIMITED BUT SIGNIFICANT HOMOLOGY WITH GLUTATHIONE S-TRANSFERASES AND ONE MEMBER INDEED SHOWS INVITRO GST ACTIVITY
PROTEINS ENCODED BY AN AUXIN-REGULATED GENE FAMILY OF TOBACCO SHARE LIMITED BUT SIGNIFICANT HOMOLOGY WITH GLUTATHIONE S-TRANSFERASES AND ONE MEMBER INDEED SHOWS INVITRO GST ACTIVITY
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DOI：
10.1007/bf00023595
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发表时间：
1993-03-01
影响因子：
5.1
通讯作者：
VANDERZAAL, EJ
中科院分区：
文献类型：
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作者：
DROOG, FNJ;HOOYKAAS, PJJ;VANDERZAAL, EJ
已经从烟草中分离出许多对应于生长素调节的mRNA的cDNA，并发现其由三个亚家族组成的多基因家族编码。已独立地从大豆和马铃薯中分离出同源蛋白。在这里，我们报告，编码的蛋白质显示出有限的，但显着的同源性，植物和动物谷胱甘肽S-转移酶（GST，EC 2.5.1.18）。对于蛋白质NT 103，由Nt 103亚家族的成员编码，我们证明了体外GST活性。这是第一次将功能归因于这组生长素诱导蛋白或其任何同源物的成员。这一发现的影响和生长素和GST之间可能的关系进行了讨论。
A number of cDNAs corresponding to auxin-regulated mRNAs have been isolated from tobacco and found to be encoded by a multigene family consisting of three subfamilies. Homologous proteins have been isolated independently from soybean and potato. Here we report that the encoded proteins show a limited but significant homology to both plant and animal glutathione S-transferases (GST, EC 2.5.1.18). For the protein NT103, encoded by a member of the Nt103 subfamily, we demonstrate an in vitro GST activity. This is the first time a function is attributed to a member of this group of auxin-induced proteins or any of its homologues. The implications of this finding and the possible relationships between auxins and GSTs are discussed.