YSL16 Is a Phloem-Localized Transporter of the Copper-Nicotianamine Complex That Is Responsible for Copper Distribution in Rice
YSL16 Is a Phloem-Localized Transporter of the Copper-Nicotianamine Complex That Is Responsible for Copper Distribution in Rice
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DOI：
10.1105/tpc.112.103820
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发表时间：
2012-09-01
期刊：
影响因子：
11.6
通讯作者：
Ma, Jian Feng
中科院分区：
文献类型：
--
作者：
Zheng, Luqing;Yamaji, Naoki;Ma, Jian Feng
Cu是植物生长的必需元素，但其在植物内分布和再分布的分子机制尚不清楚。在此，我们报道黄色条纹状 16 (YSL16) 参与水稻 (Oryza sativa) 中的铜分布和再分布。水稻YSL16在营养生长阶段在根、叶和未伸长节中表达，在生殖阶段在上部节中高表达。 YSL16在节的韧皮部和叶的维管组织中表达。敲除该基因会导致营养阶段老叶中的铜浓度较高，但较年轻的叶中的铜浓度较低。在繁殖阶段，剑叶和稻壳中的铜浓度较高，但糙米中的铜含量较低，导致敲除品系的育性显着降低。 Cu-65 同位素标记实验表明，突变体失去了将铜烟酰胺从老叶运输到幼叶以及从旗叶运输到圆锥花序的能力。水稻 YSL16 在酵母中转运铜-烟酰胺复合物。综上所述，我们的结果表明 Os-YSL16 是一种铜烟酰胺转运蛋白，需要通过韧皮部转运将铜转运至发育中的幼嫩组织和种子。
Cu is an essential element for plant growth, but the molecular mechanisms of its distribution and redistribution within the plants are unknown. Here, we report that Yellow stripe-like16 (YSL16) is involved in Cu distribution and redistribution in rice (Oryza sativa). Rice YSL16 was expressed in the roots, leaves, and unelongated nodes at the vegetative growth stage and highly expressed in the upper nodes at the reproductive stage. YSL16 was expressed at the phloem of nodes and vascular tissues of leaves. Knockout of this gene resulted in a higher Cu concentration in the older leaves but a lower concentration in the younger leaves at the vegetative stage. At the reproductive stage, a higher Cu concentration was found in the flag leaf and husk, but less Cu was present in the brown rice, resulting in a significant reduction in fertility in the knockout line. Isotope labeling experiments with Cu-65 showed that the mutant lost the ability to transport Cu-nicotianamine from older to younger leaves and from the flag leaf to the panicle. Rice YSL16 transported the Cu-nicotianamine complex in yeast. Taken together, our results indicate that Os-YSL16 is a Cu-nicotianamine transporter that is required for delivering Cu to the developing young tissues and seeds through phloem transport.