Microfilament accumulation and the transport of amino acids and glucose in adult rat hepatocytes cultured on collagen gel/nylon mesh.
Microfilament accumulation and the transport of amino acids and glucose in adult rat hepatocytes cultured on collagen gel/nylon mesh.
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在胶原凝胶/尼龙网上培养的成年大鼠肝细胞中微丝的积累以及氨基酸和葡萄糖的转运。
DOI：
10.1016/0006-291x(81)91889-1
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发表时间：
1981
影响因子：
3.1
通讯作者：
Pitot,HC
中科院分区：
文献类型：
--
作者：
Mak,WW;Pitot,HC
α-氨基异丁酸在培养肝细胞中的转运具有温度和能量依赖性，而2-脱氧-D-葡萄糖的转运不具有能量依赖性。在胶原凝胶/尼龙网片上的肝细胞早期培养物（第2天）中，细胞含有很少的微丝，并且氨基酸和葡萄糖的转运比含有微丝的顶端广泛积累的肝细胞晚期培养物（第6天）多5-7倍。细胞松弛素D对第2天肝细胞培养物中氨基酸和葡萄糖的转运几乎没有影响，但在第6天培养物中增强了这两种化合物的转运。这些结果表明，在培养的肝细胞中的微丝积累抑制氨基酸和葡萄糖的转运。
Transport of α-aminoisobutyric acid in cultured hepatocytes is temperature- and energy-dependent, whereas transport of 2-deoxy-D-glucose is not energy-dependent. In early cultures of hepatocytes (day 2) on a collagen gel/nylon mesh, the cells contain few microfilaments and the transport of amino acids and glucose is 5–7 times more than in late cultures of hepatocytes (day 6) which contain an apical, extensive accumulation of microfilaments. Cytochalasin D has little effect on the transport of amino acids and glucose in day 2 cultures of hepatocytes, but enhances transport of both compounds in day 6 cultures. These findings suggest that the microfilament accumulation in cultured hepatocytes inhibits transport of amino acids and glucose.