An IMS-IMS analogue of MS-MS
An IMS-IMS analogue of MS-MS
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DOI：
10.1021/ac051060w
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发表时间：
2006-06-15
影响因子：
7.4
通讯作者：
Clemmer, David E.
中科院分区：
文献类型：
--
作者：
Koeniger, Stormy L.;Merenbloom, Samuel I.;Clemmer, David E.
介绍了一种采用多场漂移管/离子漏斗设计的新型离子迁移率/质谱仪的研制。在该仪器中，可以根据漂移管内碰撞激活之前的迁移率差异来解析和选择离子混合物中的单个组分。在额外的碰撞活化和MS分析之前，产生的碎片离子可以再次分散在第二离子迁移率谱(IMS)区域中。结果是IMS-IMS类似于MS-MS。在这里，我们描述了初步的仪器设计和实验方法。我们通过研究高度特征化的缓激肽和泛素系统来说明这一方法。缓激肽的迁移率分辨碎片离子表明，b型离子是很容易辨别的碎片，因为它们以两种易分辨的结构类型存在。简要讨论了目前的局限性和未来的发展方向。
The development of a new ion mobility/mass spectrometry instrument that incorporates a multifield drift tube/ion funnel design is described. In this instrument, individual components from a mixture of ions can be resolved and selected on the basis of mobility differences prior to collisional activation inside the drift tube. The fragment ions that are produced can be dispersed again in a second ion mobility spectrometry (IMS) region prior to additional collisional activation and MS analysis. The result is an IMS-IMS analogue of MS-MS. Here, we describe the preliminary instrumental design and experimental approach. We illustrate the approach by examining the highly characterized bradykinin and ubiquitin systems. Mobility-resolved fragment ions of bradykinin show that b-type ions are readily discernible fragments, because they exist as two easily resolvable structural types. Current limitations and future directions are briefly discussed.