A new chemical probe for the detection of the cancer-linked galectin-3
A new chemical probe for the detection of the cancer-linked galectin-3
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DOI：
10.1039/b611050a
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发表时间：
2006-01-01
影响因子：
3.2
通讯作者：
Pieters, Roland J.
中科院分区：
文献类型：
--
作者：
Ballell, Lluis;van Scherpenzeel, Monique;Pieters, Roland J.
开发了一种化学探针，用于检测新出现的癌症标志物半乳糖凝集素-3。探针含有二苯甲酮部分，其在结合和照射后将自身共价连接到蛋白质。通过“点击”化学引入荧光标记允许标记的蛋白质在凝胶中可视化。使用探针，显示了半乳糖凝集素-3在蛋白质混合物中的选择性可视化，甚至在细胞裂解物中也显着。
A chemical probe was developed for the detection of the emerging cancer marker galectin-3. The probe contains a benzophenone moiety which covalently attaches itself to the protein upon binding and irradiation. Introduction of a fluorescent label via 'click' chemistry allows the labelled proteins to be visualized in a gel. With the probe, selective visualization of galectin-3 in protein mixtures was shown and remarkably even in cell lysates.