Body mass, index, body composition, and leptin at onset of puberty in male and female rats after intrauterine growth retardation and after early postnatal food restriction
Body mass, index, body composition, and leptin at onset of puberty in male and female rats after intrauterine growth retardation and after early postnatal food restriction
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DOI：
10.1203/00006450-200110000-00009
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发表时间：
2001-10-01
影响因子：
3.6
通讯作者：
Delemarre-van de Waal, HA
中科院分区：
文献类型：
--
作者：
Engelbregt, MJT;van Weissenbruch, MM;Delemarre-van de Waal, HA
在这项研究中，我们研究了在青春期开始时，在宫内发育迟缓（IUGR），出生后的食物限制（FR），并控制雄性和雌性大鼠的身体成分。IUGR是通过结扎子宫动脉的妊娠d 17和FR的每母从出生后d 2至断奶（d 24）的窝扩大到20只幼崽。我们将青春期的开始定义为雄性大鼠龟头包皮分离和雌性大鼠阴道张开。我们计算了体重指数，用双能X线吸收法测量了身体成分，并测量了血清中的瘦素浓度。据以前的报道，早期营养不良，无论是在妊娠后期或出生后立即，在IUGR和FR雄性大鼠和IUGR雌性大鼠的青春期延迟的结果，但不是在FR雌性大鼠。在IUGR雄性大鼠在龟头包皮分离和IUGR雌性大鼠在阴道口没有发现差异，体重指数，身体成分，瘦素水平与对照组相比。FR雄性大鼠在龟头包皮分离时脂肪百分比和血清瘦素浓度显著较低。FR雌性大鼠的体重指数、脂肪百分比和阴道口血清瘦素浓度显著降低。我们的结论是，青春期的大鼠的发病是不依赖于一定比例的体脂或一定阈值的循环水平的瘦素和食物剥夺在不同的“关键”的时间段出生后的生活中的结果在不同的影响。
In this study we examined the body composition at onset of puberty in intrauterine growth retarded (IUGR), postnatal food restricted (FR), and control male and female rats. IUGR was induced by ligation of the uterine artery on d 17 of gestation and FR by litter enlargement to 20 pups per mother from d 2 after birth until weaning (d 24). We defined onset of puberty as balanopreputial separation in male rats and vaginal opening in female rats. We calculated body mass index, measured body composition with dual-energy x-ray absorptiometry, and measured leptin concentrations in serum. It was reported previously that early malnutrition, either during late gestation or immediately postnatally, results in a delayed onset of puberty in IUGR and FR male rats and in IUGR female rats, but not in FR female rats. In IUGR male rats at balanopreputial separation and in IUGR female rats at vaginal opening no differences were found in body mass index, body composition, and leptin levels compared with controls. FR male rats had a significantly lower percentage of fat and serum leptin concentrations at balanopreputial separation. FR female rats had a significantly lower body mass index, percentage of fat, and serum leptin concentrations at vaginal opening. We conclude that the onset of puberty in the rat is not dependent on a certain percentage of body fat or a certain threshold of circulating levels of leptin and that food deprivation during different "critical" time periods around birth results in different effects in later life.