YHV-protease dsRNA inhibits YHV replication in Penaeus monodon and prevents mortality
YHV-protease dsRNA inhibits YHV replication in Penaeus monodon and prevents mortality
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DOI：
10.1016/j.bbrc.2005.12.186
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发表时间：
2006-03-10
影响因子：
3.1
通讯作者：
Panyim, S
中科院分区：
文献类型：
--
作者：
Yodmuang, S;Tirasophon, W;Panyim, S
黄头病毒感染养殖对虾，造成严重的死亡，造成巨大的经济损失。血淋巴注射与YHV基因组蛋白酶基序对应的dsRNA（pro）可完全抑制YHV的复制。dsRNA的作用持续至少5天。注射序列无关的dsRNA（gfp）或dsRNA（TSV-pol）也导致YHV复制的抑制，但程度相对较低。监测虾死亡率10天，当超过90%未接受dsRNA的虾在8dpi内死亡时。然而，接受dsRNA（pro）的那些没有显示死亡。在接受dsRNA（gfp）或dsRNA（TSV-pol）的虾中观察到部分死亡。因此，斑节对虾对YHV感染具有序列特异性保护，除了序列非依赖性保护之外，最有可能通过RNAi途径。提出了dsRNA诱导对虾抗病毒免疫的两条途径，即序列非依赖性途径和序列依赖性途径。(c)2005年爱思唯尔公司All rights reserved.
Yellow head virus infects cultured shrimps and causes severe mortality resulting in a great economic loss. Haemolymph injection of dsRNA(pro) corresponding to the protease motif of YHV genome resulted in a complete inhibition of YHV replication. The effect of dsRNA lasted for at least 5 days. Injecting sequence-unrelated dsRNA(gfp) or dsRNA(TSV-pol) also resulted in an inhibition of YHV replication but at a comparatively Much less extent. Shrimp mortality was monitored for 10 days when more than 90% shrimps receiving no dsRNA died within 8 dpi. However, those receiving dsRNA(pro) showed no mortality. A partial mortality was observed among the shrimps receiving dsRNA(gfp) or dsRNA(TSV-pol). Thus, Penaeus monodon possesses the sequence-specific protection to YHV infection, most likely through the RNAi pathways in addition to sequence-independent protection. It gives a new notion that dsRNA induction of antiviral immunity in shrimp goes through two pathways, sequence-independent and sequence-dependent. (c) 2005 Elsevier Inc. All rights reserved.