Development of a transposon mutagenesis system in the oral spirochete Treponema denticola.
Development of a transposon mutagenesis system in the oral spirochete Treponema denticola.
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口腔螺旋体齿垢密螺旋体转座子诱变系统的开发。
DOI：
10.1128/aem.01424-08
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发表时间：
2008
影响因子：
4.4
通讯作者：
Li,Chunhao
中科院分区：
文献类型：
--
作者：
Yang,Yu;Stewart,PhilipE;Shi,Xiaoguang;Li,Chunhao
在这里，我们报告了成功的转座子突变口腔螺旋体齿密螺旋体。一种改良的希玛尔转座子，包括一种新的抗生素选择盒Fort。齿状突变体产生了影响细胞分裂、运输和趋化等过程的突变。这种随机突变系统应该有助于对这种螺旋体的生物学和发病机制的研究，这种螺旋体与人类牙周疾病有关。
Here, we report successful transposon mutagenesis in the oral spirocheteTreponema denticola. A modifiedHimar1transposon, including a new antibiotic selection cassette forT. denticola, generated mutations affecting cell division, transport, and chemotaxis, among other processes. This random mutagenesis system should facilitate research on the biology and pathogenesis of this spirochete, which is associated with human periodontal diseases.