Integration of a flow-type chemiluminescence detector on a glass electrophoresis chip
Integration of a flow-type chemiluminescence detector on a glass electrophoresis chip
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DOI：
10.1016/j.talanta.2004.04.024
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发表时间：
2004-11-15
期刊：
影响因子：
6.1
通讯作者：
Yamada, M
中科院分区：
文献类型：
--
作者：
Su, RG;Lin, JM;Yamada, M
研制了一种集成流动式化学发光检测池的玻璃电泳微芯片。芯片模式为双T型进样分离与Y型化学发光检测器相结合。双T几何结构允许高效率的样品注射和样品塞尺寸的几何定义。用Y支管作为化学发光试剂通道，用自制的微泵输送化学发光试剂。采用双[(2，4，6-三氯苯基)]草酸-H_2O_2-CL体系测定丹磺酰氨基酸。在该芯片上成功地实现了丹磺酰苯丙氨酸和肌氨酸的分离，并在250 S范围内进行了检测，由于用CL试剂对样品进行了严格的稀释，并及时清除了反应区的废液，丹磺酰苯丙氨酸和肌氨酸的检出限(S/N=3)分别达到了2.8和3.2微米。(C)2004爱思唯尔B.V.保留所有权利。
A glass electrophoresis microchip integrated a flow-type chemluminescence (CL) detection cell has been developed and evaluated. The chip pattern is a double-T-type electrophoretic sample injection and separation combining with a Y-type chemiluminecent detector. The double-T geometry allows for high-efficiency sample injection and geometric definition of sample plug size. The branch of Y was used as CL reagent channel, and the CL reagent was delivered by a tab-made micropump. Bis[(2,4,6-trichlorophenyl)]oxalate-H2O2 CL system was employed to detect dansyl amino acids. On this microchip, dansyl-phenylalanine and -sarcosine were successfully separated by electrophoresis and detected within 250 s. The detection limits (S/N = 3) of dansyl-phenylalanine and -sarcosine could reach to 2.8 and 3.2 muM, respectively, due to the vigorous dilution of sample with CL reagent and timely removal of the waste solution from reaction area. (C) 2004 Elsevier B.V. All rights reserved.