Transglutaminase-catalyzed preparation of crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen composite membrane for postsurgical peritoneal adhesion prevention
Transglutaminase-catalyzed preparation of crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen composite membrane for postsurgical peritoneal adhesion prevention
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DOI：
10.1016/j.carbpol.2018.08.065
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发表时间：
2018-12-01
影响因子：
11.2
通讯作者：
Shi, Xianai
中科院分区：
文献类型：
--
作者：
Cai, Xianqun;Hu, Shengxue;Shi, Xianai
腹膜粘连是盆腔和腹部手术后常见的并发症，可导致慢性腹痛、肠梗阻、器官损伤和女性不孕。生物可降解聚合物膜已被建议作为物理屏障，以防止腹膜粘连。本研究以转谷氨酰胺酶（TGase）为催化剂，制备了羧甲基壳聚糖/羧甲基纤维素/胶原（CMCS/CMCL/COL）复合防粘连膜，其中CMCS、CMCL和COL的比例为40/40/20、35/35/30、25/25/50。TGase交联后，复合防粘连膜的力学性能和生物降解性得到了提高。同时，体外细胞培养实验证明了抗粘连膜具有良好的细胞相容性。将抗粘连膜按25/25/50的比例植入大鼠盲肠和腹膜间，进行大体观察、组织学检查和炎性因子测定。结果表明，防粘连膜能显著防止腹腔粘连，且免疫原性可忽略。因此，TGase交联的复合膜具有良好的抗粘连效果，生物相容性好，抗原性低，可作为预防腹膜粘连的屏障。
Peritoneal adhesion is a general complication following pelvic and abdominal surgery, which may lead to chronic abdominal pain, bowel obstruction, organ injury, and female infertility. Biodegradable polymer membranes have been suggested as physical barriers to prevent peritoneum adhesion. In this work, a transglutaminase (TGase)-catalyzed crosslinked carboxymethyl chitosan/carboxymethyl cellulose/collagen (CMCS/CMCL/COL) composite anti-adhesion membrane with various proportions of CMCS, CMCL, and COL (40/40/20, 35/35/30, 25/25/50) was developed. After crosslinking by TGase, the composite anti-adhesion membranes shown enhanced mechanical properties and improved biodegradability. Meanwhile, the high cytocompatibility of anti-adhesion membranes was proved by in vitro cell culture study. Moreover, the anti-adhesion membrane with the proportion of 25/25/50 was implanted between the artificially defected cecum and peritoneal wall in rats and following by general observation, histological examination, and inflammatory factors assay. The results indicated that the anti-adhesion membrane can significantly prevent peritoneal adhesion with negligible immunogenicity. Therefore, the composite membrane crosslinked by TGase had satisfactory anti-adhesive effects with high biocompatibility and low antigenicity, which could be used as a preventive barrier for peritoneal adhesion.