Widespread occurrence of argyrophilic glial inclusions in Parkinson's disease
Widespread occurrence of argyrophilic glial inclusions in Parkinson's disease
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DOI：
10.1046/j.1365-2990.2001.00345.x
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发表时间：
2001-10-01
影响因子：
5
通讯作者：
Sobue, G
中科院分区：
文献类型：
--
作者：
Hishikawa, N;Hashizume, Y;Sobue, G
在帕金森病（PD）患者的脑中检查了嗜银神经胶质包涵体，其对α-突触核蛋白呈化学阳性，但对tau蛋白呈阴性。尸检的10个人谁死于PD，两个偶然的路易体病的情况下和5个年龄匹配的个人，其死亡原因是非神经系统疾病的大脑进行了研究，组织病理学，Gallyas-Braak染色，免疫组化，抗α-突触核蛋白抗体，抗泛素，抗酪氨酸羟化酶。所有尸检PD大脑显示出显着数量的嗜银胶质细胞夹杂物，但没有胶质细胞夹杂物被发现在对照组的大脑。包涵体不仅存在于黑质、蓝斑、迷走神经背核等神经元缺失和胶质增生的区域，也存在于大脑皮质、大脑白色质、纹状体、苍白球、丘脑、小脑、脊髓等无神经元缺失和胶质增生的区域。PD脑中胶质细胞包涵体的分布和密度因情况而异，但在大脑皮层中，胶质细胞包涵体的数量与路易体的数量有相当好的相关性。神经胶质包涵体的分布模式也表现出惊人的相似，儿茶酚胺能神经元和纤维。α-突触核蛋白在胶质细胞中的异常积聚比神经元丢失更广泛，并且似乎是PD的重要病理特征。
Argyrophilic glial inclusions, which are immunohistochemically positive for alpha -synuclein but negative for tau protein, were examined in the brain of Parkinson's disease (PD) patients. Autopsied brains of 10 individuals who died from PD, of two incidental Lewy body disease cases and of five age-matched individuals whose deaths were caused by non-neurological diseases were studied, histopathologically, by Gallyas-Braak staining and, Immunohistochemically, with anti-alpha -synuclein antibody, anti-ubiquitin, and anti-tyrosine hydroxylase. All postmortem PD brains showed a significant number of argyrophilic glial inclusions, but no glial inclusions were found in control brains. The inclusions were found not only in the regions showing neuronal loss and gliosis, such as the substantia nigra, locus ceruleus and dorsal vagal nucleus, but also in regions without neuronal loss and gliosis, such as the cerebral cortex, cerebral white matter, striatum, globus pallidus, thalamus, cerebellum and spinal cord. The distribution and density of glial inclusions in PD brains varied from case to case but, in the cerebral cortex, the number of glial inclusions were fairly well correlated with the number of Lewy bodies. The distribution pattern of glial inclusions also showed a striking resemblance to that of catecholaminergic neurones and fibres. The abnormal accumulation of alpha -synuclein in glial cells was more widespread than neurone loss, and appears to be an important pathological feature of PD.