Target MicroRNA-Responsive DNA Hydrogel-Based Surface-Enhanced Raman Scattering Sensor Arrays for MicroRNA-Marked Cancer Screening
Target MicroRNA-Responsive DNA Hydrogel-Based Surface-Enhanced Raman Scattering Sensor Arrays for MicroRNA-Marked Cancer Screening
复制标题
基于 MicroRNA 响应的 DNA 水凝胶表面增强拉曼散射传感器阵列，用于 MicroRNA 标记的癌症筛查
DOI：
10.1021/acs.analchem.9b04606
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发表时间：
2020-02-04
影响因子：
7.4
通讯作者：
Li, Jishan
中科院分区：
文献类型：
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作者：
Si, Yanmei;Xu, Lan;Li, Jishan
基于靶向microRNA（miRNA）响应性DNA水凝胶，开发了一种新型的表面增强拉曼散射（Sers）传感器阵列，该阵列具有9个传感单元，可以检测一个样品中的多种癌症相关miRNA。首先在每个传感器单元中形成目标miRNA响应的DNA水凝胶，以实现基于DNA水凝胶的Sers传感器阵列的构建。最初，由于形成的DNA水凝胶对链霉亲和素（SA）修饰的传感器单元的阻断，Sers标签（生物素/4-巯基苯甲腈官能化的AuAg合金纳米颗粒（B/M-AuAgNPs））不能穿过水凝胶并结合到SA修饰的传感器表面;因此，不能观察到明显的拉曼信号。在引入目标miRNA后，相应传感器单元的DNA水凝胶被相应地分解，并且Sers标签能够穿过水凝胶被捕获到SA修饰的检测表面上，从而产生强的拉曼信号并检测目标miRNA。在清洁缓冲液条件下以及在血清中验证该测定法。这种靶向miRNA响应的DNA水凝胶Sers传感器阵列在通过血液miRNA测量进行癌症分型方面具有诱人的应用前景。
On the basis of a target microRNA (miRNA)-responsive DNA hydrogel, a novel surface-enhanced Raman scattering (SERS) sensor array with nine sensor units that can detect multiple cancer-related miRNAs in one sample was developed. The target miRNA-responsive DNA hydrogel was first formed in each sensor unit to realize the construction of the DNA hydrogel-based SERS sensor array. Initially, because of the blocking of the streptavidin (SA)-modified sensor units by the formed DNA hydrogel, the SERS tags (biotin/4-mercaptobenzonitrile-functionalized AuAg alloy nanoparticles (B/M-AuAgNPs)) could not pass through the hydrogel and bind to the SA-modified sensor surface; thus, obvious Raman signals could not be observed. After the introduction of the target miRNA, DNA hydrogels of the corresponding sensor unit were disintegrated accordingly, and SERS tags were able to pass through the hydrogel to be captured onto the SA-modified detection surface, thus resulting in strong Raman signals and the detection of target miRNA. The assay is validated under clean buffer conditions as well as in serum. This target miRNA-responsive DNA hydrogel-based SERS sensor array has attractive application prospects in cancer typing via blood miRNA measurements.