Spray congealing as innovative technique for enzyme encapsulation
Spray congealing as innovative technique for enzyme encapsulation
复制标题
喷雾凝结作为酶封装的创新技术
DOI：
10.1002/jctb.5339
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发表时间：
2018
影响因子：
3.4
通讯作者：
Kragl U.
中科院分区：
文献类型：
--
作者：
Engelmann;Kragl U.
喷雾凝固法是一种新型的生物催化剂固定化技术。固定化通过熔化的载体和酶的混合物的雾化来实现，该混合物由于温度降低而重新固化。该方法不需要有机溶剂，制备后的下游处理也不困难。对于不同基质的包封，选择熔点低于86 ℃且具有环境友好性质的长链醇和甘油基衍生物，并测试它们对酶包封的适用性。研究漆酶（EC 1.10.3.2，Novozym 51003，来自嗜热毁丝霉（Myceliophthora thermophila））的固定化，并表征所产生的微粒。所得颗粒的平均粒径约为40 μm，球形表面，根据所用基质的不同，具有不同的结构。用该漆酶进行喷雾冻凝，产率达80%，残酶率达56%。此外，四个连续的反应，实现了与鲸蜡醇为载体，并保留了20%.CONCLUSION鲸蜡醇被认为是最合适的载体，用于固定化的漆酶，由于易于操作和高产率。与其他载体相比，该载体的活性保留率最高，实现了生物催化剂的循环利用。© 2017化学工业协会
BACKGROUNDSpray congealing is investigated as a new innovative immobilization technique for biocatalysts. The immobilization is realized by atomization of a mixture of a melted carrier and the enzyme, which is re‐solidifyed due to temperature decrease. This method needs neither organic solvents, nor difficult downstream processing after preparation. For the encapsulation of different matrices, long chain alcohols and glyceryl derivatives, with melting points below 86 °C and environmentally friendly properties, are chosen and tested for their suitability for enzyme encapsulation.RESULTSThe immobilization of a laccase (EC 1.10.3.2, Novozym 51003, fromMyceliophthora thermophila) was investigated and the produced microparticles were characterized. The obtained particles showed a mean particle size around 40 μm and a spherical surface with different structures depending on the used matrix. Spray congealing with the laccase reached yields of 80% and residual activities up to 56%. Furthermore, four consecutive reactions were realized with cetyl alcohol as carrier and an activity of 20% was retained.CONCLUSIONCetyl alcohol was found to be the most appropriate carrier for the immobilization of the investigated laccase, due to easy handling and high yields. In comparison with the other tested carriers, the best retained activities were obtained and recycling of the biocatalyst was realized. © 2017 Society of Chemical Industry
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影响因子：
4.4
作者：
Engelmann, Claudia;Illner, Sabine;Kragl, Udo
通讯作者：
Kragl, Udo
适用于喷雾凝结的脂质赋形剂的粘度-温度关系：推导与粒径具有良好相关性的流变参数
DOI：
--
发表时间：
2016
期刊：
影响因子：
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作者：
Priscilla Chui Hong Wong;P. Heng;L. Chan
通讯作者：
L. Chan
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DOI：
--
发表时间：
2002
期刊：
Journal of Pharmacy and Science
影响因子：
--
作者：
N. Passerini;B. Perissutti;M. Moneghini;D. Voinovich;B. Albertini;C. Cavallari;L. Rodriguez
通讯作者：
L. Rodriguez
DOI：
--
发表时间：
2012
期刊：
影响因子：
--
作者：
M. Constantin;J. Conrad;U. Beifuss
通讯作者：
U. Beifuss
影响因子：
2.2
作者：
D. L. Pedroso;M. Dogenski;M. Thomazini;R. Heinemann;C. Fávaro
通讯作者：
C. Fávaro