Characterization of the 12S globulin complex of Brassica napus. Evolutionary relationship to other 11-12S storage globulins.
Characterization of the 12S globulin complex of Brassica napus. Evolutionary relationship to other 11-12S storage globulins.
复制标题
甘蓝型油菜 12S 球蛋白复合物的表征。
DOI：
10.1111/j.1432-1033.1991.tb15857.x
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
L. Rask
中科院分区：
文献类型：
--
作者：
Staffan Sjödahl;J. Rödin;L. Rask
十字花科素（12S 球蛋白）是甘蓝型油菜（油菜）中的主要种子蛋白。它在种子发育过程中合成，由六个亚基对组成。这些对中的每一对都是作为包含一个 α 链和一个 β 链的前体合成的。至少存在三种不同的前体 (P1-3)，产生四种不同的成熟亚基 (cru1-4)。从种子 mRNA cDNA 文库中分离出几个十字花科素克隆。将这些克隆推导的氨基酸序列与纯化的十字花科素链和肽的氨基酸序列进行比较，确定它们编码cru2/3和cru4亚基。根据两个重叠 cDNA 克隆推导的氨基酸序列，cru4 亚基的前体由 465 个氨基酸残基组成。分别来自欧洲油菜和拟南芥的十字花科素和十字花科素相关序列的比较表明，在进化早期，十字花科仅拥有两种类型的11-12S球蛋白基因，就像现在的豆科植物一样。
Cruciferin (12S globulin) is the major seed protein in Brassica napus (oil seed rape). It is synthesized during seed development and consists of six subunit pairs. Each of these pairs is synthesized as a precursor containing one alpha and one beta chain. At least three different precursors exist (P1-3), giving rise to four different mature subunits (cru1-4). Several cruciferin clones were isolated from a seed mRNA cDNA library. Comparison of the deduced amino acid sequences of these clones to amino acid sequences of purified cruciferin chains and peptides identified them as coding for cru2/3 and cru4 subunits. From the amino acid sequences deduced from two overlapping cDNA clones, the precursor of the cru4 subunit was shown to consist of 465 amino acid residues. Comparison of cruciferin and cruciferin-related sequences from B. napus and Arabidopsis thaliana, respectively, suggested that early during evolution the Brassicaceae family only possessed two types of 11-12S globulin genes, like the present-day Fabaceae.