Label-free and non-enzymatic detection of DNA based on hybridization chain reaction amplification and dsDNA-templated copper nanoparticles
Label-free and non-enzymatic detection of DNA based on hybridization chain reaction amplification and dsDNA-templated copper nanoparticles
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基于杂交链式反应扩增和 dsDNA 模板铜纳米粒子的 DNA 无标记和非酶检测
DOI：
10.1016/j.aca.2014.04.006
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发表时间：
2014-05-27
影响因子：
6.2
通讯作者：
Liu, Pei
中科院分区：
文献类型：
--
作者：
Song, Chunxia;Yang, Xiaohai;Liu, Pei
通过使用杂交链式反应 (HCR) 和双链 DNA 模板铜纳米颗粒 (CuNP)，开发了一种用于检测 DNA 的无标记和非酶扩增荧光方法。首先，通过生物素和链霉亲和素的相互作用将生物素化的捕获DNA探针固定在链霉亲和素修饰的珠子上。然后，目标DNA与捕获DNA探针杂交，形成具有粘性末端的杂交DNA。当两个发夹探针共存时，粘性末端触发 HCR 过程和 dsDNA 聚合物的形成。随后，双链 DNA 聚合物被用作合成具有优异荧光特性的 CuNP 的模板，从而提供无标记、非酶信号响应。同时，荧光传感依赖于目标DNA触发的HCR，这使得该方法对单碱基错配序列具有高选择性。这项工作中开发的概念和方法在生物和医学应用中的 DNA 定量检测方面显示出巨大的前景。 (C) 2014 Elsevier B.V. 保留所有权利。
A label-free and non-enzymatic amplification fluorescent method for detection of DNA has been developed by using hybridization chain reaction (HCR) and dsDNA-templated copper nanoparticles (CuNPs). First, the biotinylated capture DNA probes were immobilized on the streptavidin-modified beads through the interaction of biotin and streptavidin. Then, target DNA hybridized with the capture DNA probes, which formed a hybridized DNA with sticky end. The sticky end triggered the HCR process and formation of dsDNA polymers while two hairpin probes coexisted. Subsequently, the dsDNA polymers were employed as template for synthesis of CuNPs with excellent fluorescent properties, which provided a label-free, non-enzymatic signal response. Meanwhile, the fluorescence sensing depended on the target DNA triggered HCR, which render this method a high selectivity against single-base mismatch sequences. The concept and methodology developed in this work show great promise in the quantitative detection of DNA in biological and medical applications. (C) 2014 Elsevier B.V. All rights reserved.