AMPLIFICATION OF THE NA+-ATPASE OF STREPTOCOCCUS-FAECALIS AT ALKALINE PH

AMPLIFICATION OF THE NA+-ATPASE OF STREPTOCOCCUS-FAECALIS AT ALKALINE PH
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DOI:
10.1016/0014-5793(90)80654-2
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发表时间:
1990-02-12
期刊:
影响因子:
3.5
通讯作者:
IGARASHI, K
IGARASHI, K
中科院分区:
生物学3区
文献类型:
--
作者:
KAKINUMA, Y;IGARASHI, K

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粪链球菌的Na+‐atp酶活性受培养基pH的影响。在pH为9.5时生长的细胞中,抗质子载体Na+挤压和KtrII (Na+‐atp酶对K+的活性摄取)的活性最高,而在pH为6.0时生长的细胞中活性最低。在pH 7.5下生长的细胞中,它们被适度观察到。pH值为9.5的细胞的atp酶活性比pH值为6.0的细胞高约4倍。因此,在这种生物体中,Na+‐atp酶的扩增在碱性条件下是显著的,可能是通过细胞质Na+水平的增加作为信号。
The Na+‐ATPase activity ofStreptococcus faecaliswas influenced by the medium pH. Activities of the protonophore‐resistant Na+extrusion and the KtrII (active K+uptake by the Na+‐ATPase) were maximal in the cells grown at pH 9.5, and were minimal in those grown at pH 6.O. In the cells grown at pH 7.5, they were moderately observed. The Na+‐stimulated ATPase activity of the cells grown at pH 9.5 was about 4‐fold higher than that of the cells grown at pH 6.O. Thus, amplification of the Na+‐ATPase is remarkable at alkaline pH in this organism, possibly by an increase of the cytoplasmic Na+level as a signal.