Neuroregeneration in the Nucleus Ambiguus After Recurrent Laryngeal Nerve Avulsion in Rats
Neuroregeneration in the Nucleus Ambiguus After Recurrent Laryngeal Nerve Avulsion in Rats
复制标题
大鼠喉返神经撕脱后模糊核的神经再生
DOI：
10.1177/0003489414524170
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发表时间：
2014-07-01
影响因子：
1.4
通讯作者：
Yang, Wei Wei
中科院分区：
文献类型：
--
作者：
Zhao, Wan;Xu, Wen;Yang, Wei Wei
目的：目的是探讨同侧喉返神经（RLN）撕脱后疑核（NA）内神经再生、新生细胞的来源以及神经元和胶质细胞的增殖。方法：将CM-Dil注射于左侧脑室。45只成年大鼠进行左侧喉返神经撕脱伤，而9只大鼠作为对照。腹腔注射5-溴-2-脱氧尿苷（BrdU）。在喉返神经损伤后不同时间点对脑干进行神经元定量和免疫组织化学分析。结果如下：喉返神经撕脱伤后，CM-Dil标记的神经前体细胞（NPC）迁移到同侧NA，并分化为星形胶质细胞，但不分化为神经元。在NA中，神经元细胞重新表达巢蛋白。只有少数神经元和神经胶质细胞的NA显示BrdU免疫反应。结论：喉返神经撕脱伤后，第四脑室或中央管室管膜层的NPCs被激活，迁移到NA的损伤处，并分化为星形胶质细胞。新生的神经干细胞可能来源于成熟的神经元。这两种细胞类型在NA的存在可能在修复RLN损伤中发挥作用。
Objectives: The objective was to investigate neuroregeneration, the origins of newborn cells and the proliferation of neuronal and glial cells in the nucleus ambiguus (NA) after ipsilateral recurrent laryngeal nerve (RLN) avulsion. Methods: All of the animals received a CM-Dil injection in the left lateral ventricle. Forty-five adult rats were subjected to a left RLN avulsion injury, while 9 rats were used as controls. 5-Bromo-2-deoxyuridine (BrdU) was injected intraperitoneally. Neuron quantification and immunohistochemical analysis were performed in the brain stems at different time points after RLN injury. Results: After RLN avulsion, CM-Dil labeled neural progenitor cells (NPCs) migrated to the ipsilateral NA and differentiated into astrocytes but not into neurons. In the NA, the neuronal cells re-expressed nestin. Only a small number of neuronal and glial cells in the NA showed BrdU immunoreactivity. Conclusions: After RLN avulsion, the NPCs in the ependymal layer of the fourth ventricle or central canal are activated, migrate to the lesion in the NA and differentiate exclusively into astrocytes. The newborn neural stem cells in the NA may arise from the mature region neurons. The presence of both cell types in the NA may play a role in repairing RLN injuries.