Expression of vesicular glutamate transporters 1 and 2 in the cells of origin of the rat thalamostriatal pathway
Expression of vesicular glutamate transporters 1 and 2 in the cells of origin of the rat thalamostriatal pathway
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DOI：
10.1016/j.jchemneu.2007.08.001
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发表时间：
2008-01-01
影响因子：
2.8
通讯作者：
Lanciego, Jose L.
中科院分区：
文献类型：
--
作者：
Barroso-Chinea, Pedro;Castle, Maria;Lanciego, Jose L.
本研究的重点是囊泡谷氨酸转运蛋白1和2（VGLUT 1和VGLUT 2）的丘脑神经元引起丘脑纹状体系统的分析。而不是研究的VGLUT蛋白质的分布在丘脑纹状体终端的水平，本报告的重点是确定的VGLUT mRNA的表达内的母细胞体的丘脑神经元支配纹状体。为了这个目的，我们结合了双重原位杂交检测VGLUT I和VGLUT 2 mRNA与霍乱毒素逆行追踪。我们的研究结果表明，VGLUT2是唯一的囊泡谷氨酸转运蛋白表达在丘脑纹状体投射神经元位于中线和intralaminar核，而所有神经元从丘脑腹侧核支配纹状体表达VGLUTs，至少在mRNA水平。事实上，编码VGLUT 1和VGLUT 2的mRNA在丘脑腹侧核的神经元内显示出明显的互补亚细胞分布，引起丘脑纹状体投射。VGLUT mRNA的差异分布使我们得出结论，丘脑纹状体通路是一个双重系统，由一个优势的投影所产生的中线和intralaminar核使用VGLUT 2作为谷氨酸转运蛋白，连同另一个重要来源的纹状体传入所产生的神经元在腹侧丘脑中继核含有两种囊泡谷氨酸转运蛋白。(c)2007 Elsevier B.V.保留所有权利。
The present study is focused on the analysis of the vesicular glutamate transporters 1 and 2 (VGLUT1 and VGLUT2) used by thalamic neurons giving rise to the thalamostriatal system. Instead of studying the distribution of VGLUT proteins at the level of thalamostriatal terminals, this report is focused on identifying the expression of the VGLUT mRNAs within the parent cell bodies of thalamic neurons innervating the striatum. For this purpose, we have combined dual in situ hybridization to detect both VGLUT I and VGLUT2 mRNAs together with retrograde tracing with cholera toxin. Our results show that VGLUT2 is the only vesicular glutamate transporter expressed in thalamostriatal-projecting neurons located in the midline and intralaminar nuclei, whereas all neurons from the ventral thalamic nuclei innervating the striatum express both VGLUTs, at least at the mRNA level. Indeed, the mRNAs encoding for VGLUT1 and VGLUT2 displayed a sharp complementary subcellular distribution within neurons from the ventral thalamic nuclei giving rise to thalamostriatal projections. The differential distribution of VGLUT mRNAs lead us to conclude that the thalamostriatal pathway is a dual system, composed by a preponderant projection arising from the midline and intralaminar nuclei using VGLUT2 as the glutamate transporter, together with another important source of striatal afferents arising from neurons in the ventral thalamic relay nuclei containing both kinds of vesicular glutamate transporters. (c) 2007 Elsevier B.V. All rights reserved.