Rapid and visual detection of Listeria monocytogenes based on nanoparticle cluster catalyzed signal amplification.
Rapid and visual detection of Listeria monocytogenes based on nanoparticle cluster catalyzed signal amplification.
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DOI：
10.1016/j.bios.2016.05.100
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发表时间：
2016-12
影响因子：
12.6
通讯作者：
Lisha Zhang;R. Huang;Weipeng Liu;Hongxing Liu;Xiaoming Zhou;D. Xing
中科院分区：
文献类型：
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作者：
Lisha Zhang;R. Huang;Weipeng Liu;Hongxing Liu;Xiaoming Zhou;D. Xing
食源性病原体对全球人类健康构成重大威胁。食源性致病菌的鉴定需要快速、准确和方便。本文构建了一种纳米颗粒簇（NPC）催化的信号放大生物传感器，用于食源性致病菌的视觉检测。万古霉素（货车）是一种针对革兰氏阳性菌的糖肽类抗生素，本研究首次利用万古霉素作为分子识别剂捕获单核细胞增生李斯特菌（Listeriamonocytogenes，L.单核细胞增多症）。以Fe 3 O 4-NPC修饰的核酸适体作为信号放大纳米探针，特异性识别L.单核细胞增多症正如万古霉素和适体所识别的那样。在不同的位点上，夹心识别均表现出较好的特异性。与单个Fe 3 O 4纳米粒子（NP）相比，NPC对显色底物的显色反应表现出集体效应增强的催化活性。吸光度或颜色的变化可以代表靶标的浓度。采用基于Fe 3 O 4 NPC的信号放大方法，对L.方法的线性范围为5.4×103- 10 ~ 8 cfu/mL，检出限为5.4× 103 cfu/mL。Fe_3O_4-NPC法比Fe_3O_4-NP法更灵敏。该传感器具有灵敏度高、直观、省力等优点，在食源性致病菌检测中具有潜在的应用前景。
Foodborne pathogens pose a significant threat to human health worldwide. The identification of foodborne pathogens needs to be rapid, accurate and convenient. Here, we constructed a nanoparticle cluster (NPC) catalyzed signal amplification biosensor for foodborne pathogens visual detection. In this work, vancomycin (Van), a glycopeptide antibiotic for Gram-positive bacteria, was used as the first molecular recognition agent to captureListeriamonocytogenes(L. monocytogenes). Fe3O4NPC modified aptamer, was used as the signal amplification nanoprobe, specifically recognize to the cell wall ofL. monocytogenes. As vancomycin and aptamer recognizeL. monocytogenesat different sites, the sandwich recognition showed satisfied specificity. Compared to individual Fe3O4nanoparticle (NP), NPC exhibit collective effect-enhanced catalytic activity for the color reaction of chromogenic substrate. The change in absorbance or color could represent the concentration of target. Using the Fe3O4NPC-based signal amplification method,L. monocytogeneswhole cells could be directly assayed within a linear range of 5.4×103–108cfu/mL and a visual limit of detection of 5.4×103cfu/mL. Fe3O4NPC-based method was more sensitive than the Fe3O4NP-based method. All these attractive characteristics of highly sensitivity, visual and labor-saving, make the biosensor possess a potential application for foodborne pathogenic bacteria detection.