Genomic organization of a mouse glyceraldehyde 3-phosphate dehydrogenase gene (Gapd-s) expressed in post-meiotic spermatogenic cells.
Genomic organization of a mouse glyceraldehyde 3-phosphate dehydrogenase gene (Gapd-s) expressed in post-meiotic spermatogenic cells.
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在减数分裂后生精细胞中表达的小鼠甘油醛 3-磷酸脱氢酶基因 (Gapd-s) 的基因组结构。
DOI：
10.1002/dvg.1020160210
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Eddy,EM
中科院分区：
文献类型：
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作者：
Welch,JE;Brown,PR;O'Brien,DA;Eddy,EM
Gapds基因编码一种仅在减数分裂后的生精细胞中表达的3-磷酸甘油醛脱氢酶亚型。从小鼠基因组文库中分离出两个含有Gapd ‐ s基因的克隆。测序和限制性内切酶分析表明，该单拷贝基因包含11个外显子，跨越9596个碱基对。与鸡和人类体细胞Gapd基因的相应部分相比，Gapd-外显子和内含子的位置是保守的。启动子区不含TATA盒，但在外显子1内存在潜在的SP1识别位点。与其他TATA-less基因一样，引物延伸分析揭示了转录起始位点的一些异质性，Gapd-转录本起始于三个离散位点。北方分析表明，1.5 kbGapd-smRNA在至少三种哺乳动物的睾丸中表达，表明Gapd-s基因出现在哺乳动物进化的早期。使用GAPD缺陷型细菌，小鼠GAPD-S被证明能够作为糖酵解酶发挥作用。由于GAPD被认为是调节生精细胞糖酵解的关键酶，因此GAPD-S可能是毒理学或避孕药通过干扰糖酵解影响生育力的潜在靶点。© 1995 Wiley利斯公司
TheGapd‐sgene encodes an isoform of the glyceraldehyde 3‐phosphate dehy‐drogenase enzyme expressed only in post‐meiotic spermatogenic cells. Two clones containing theGapd‐sgene were isolated from a mouse genomic library. Sequencing and restriction enzyme analysis demonstrated that this single‐copy gene contains 11 exons and spans 9596 base pairs. The locations ofGapd‐sexons and introns are conserved when compared to the corresponding portions of the chicken and human somaticGapdgenes. The promoter region contains no TATA box, although there is a potential SP1 recognition site within exon 1. Like other TATA‐less genes, primer extension analysis reveals some heterogeneity in the site of transcription initiation withGapd‐stranscripts initiating from three discrete sites. Northern analysis demonstrated that a 1.5‐kbGapd‐smRNA is expressed in the testis in at least three mammalian orders, indicating that theGapd‐sgene appeared early in mammalian evolution. Using GAPD‐deficient bacteria, mouse GAPD‐S was shown to be capable of functioning as a glycolytic enzyme. Since GAPD has been proposed to be a key enzyme regulating glycolysis in spermatogenic cells, GAPD‐S may represent a potential target for toxicological or contraceptive agents affecting fertility by interfering with glycolysis. © 1995 Wiley‐Liss, Inc.