MAST-CELL HETEROGENEITY IN DOG SKIN
MAST-CELL HETEROGENEITY IN DOG SKIN
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DOI：
10.1002/ar.1092130402
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发表时间：
1985-01-01
影响因子：
--
通讯作者：
GOLD, WM
中科院分区：
文献类型：
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作者：
BECKER, AB;CHUNG, KF;GOLD, WM
肥大细胞颗粒的异染程度因组织固定的类型以及不同组织和物种的不同而不同。本研究试图确定狗皮肤中的肥大细胞在固定和染色特性方面是否具有异质性。我们对6只麻醉的特应性犬和1只杂种犬进行了皮肤活检。一份活检固定在福尔马林中，另一份取自平行的腹部部位，固定在碱性醋酸铅(Mota‘S溶液)中。每一次活检的相邻切片均用阿尔新蓝(1%，pH 0.5)或氯乙酸酯酶活性进行染色。在阿尔辛蓝染色切片中，在固定在福尔马林(1,836.+-)的皮肤中检测到的肥大细胞减少了三分之一。454个肥大细胞/毫米~3，平均+-。扫描电子显微镜)，而在碱性醋酸铅固定的皮肤中(2,684.+-。527个肥大细胞/mm~3)(P<；0.05)。无论使用何种固定剂，氯乙酸酯酶反应均可检测到较多的肥大细胞。我们的结论是，肥大细胞的异质性，正如不同类型的组织固定后的异色染色所证明的那样，存在于狗的皮肤中。无论固定与否，“典型”肥大细胞都会染上阿尔新蓝；然而，“非典型”肥大细胞只有在碱性醋酸铅固定后才表现出异染性。典型和非典型类型的肥大细胞均具有氯乙酸酯酶活性。
The degree of metachromasia of mast cell granules is known to vary with the type of tissue fixation and among different tissues and species. The present study sought to determine whether mast cells in dog skin are heterogeneous with respect to fixation and staining properties. We performed skin biopsies in six anesthetized, atopic dogs and one mongrel dog. One biopsy was fixed in formalin and a second, from a parallel abdominal site, was fixed in basic lead acetate (Mota''s solution). Adjacent sections from each biopsy were stained with alcian blue (1%, pH 0.5) or for chloroacetate esterase activity. In alcin blue-stained sections, one-third fewer mast cells were detected in skin fixed in formalin (1,836 .+-. 454 mast cells/mm3, mean .+-. SEM) than in skin fixed in basic lead acetate (2,684 .+-. 527 mast cells/mm3) (P < 0.05). The chloroacetate esterase reaction detected the larger number of mast cells regardless of the fixative used. We conclude that mast cell heterogeneity, as demonstrated by metachromatic staining following different types of tissue fixation, exists in dog skin. "Typical" mast cells stain with alcian blue regardless of fixation; however, "atypical" mast cells exhibit metachromasia only afer fixation in basic lead acetate. Both the typical and atypical types of mast cells have chloroacetate esterase activity.