Integration host factor (IHF) represses a Chlamydomonas chloroplast promoter in E. coli.
Integration host factor (IHF) represses a Chlamydomonas chloroplast promoter in E. coli.
复制标题
整合宿主因子 (IHF) 抑制大肠杆菌中的衣藻叶绿体启动子。
DOI：
10.1093/nar/16.8.3313
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发表时间：
1988
影响因子：
14.9
通讯作者：
Mosig,G
中科院分区：
文献类型：
--
作者：
Thompson,RJ;Mosig,G
We show that inE.coli, aChlamydomonaschloroplast promoter, PA, is repressed by Integration Host Factor (IHF). ThehimA42mutation, altering the α-subunit ofE.coliIHF, leads to over-accumulation of PAtranscriptsin vivo. This effect requires upstream chloroplast DNA sequences. DNAase I and methylatlon protection experiments show that IHF bindsin vitroto a site within PAand band-retardation shows that IHF inhibits formation of PA-E.coliRNA polymerase open complexes. We Interpret these results, together with our previous deletion analyses, to mean that inE.coli, repression of PAby IHF minimally requires both binding of IHF to a site overlapping PAand binding of one or more additional proteins, perhaps including IHF itself, to sequences upstream of PA.
