Correlation between cell killing and massive second-round superinfection by members of some subgroups of avian leukosis virus
Correlation between cell killing and massive second-round superinfection by members of some subgroups of avian leukosis virus
复制标题
禽白血病病毒某些亚群成员的细胞杀伤与大规模第二轮重复感染之间的相关性
作者：
S. Weller;A. Joy;H. Temin
亚群B、D和F的禽白血病病毒对鸡细胞具有致细胞病变性，而亚群A、C和E的病毒则不具有致细胞病变性。通过杂交和转染试验，测定了细胞中未整合的线性病毒DNA在感染致细胞病变或非致细胞病变病毒后不同时间的量。感染后不久，在细胞病变性禽白血病病毒感染的细胞中，存在未整合的线性病毒DNA的瞬时积累。到感染后10天，大多数未整合的病毒DNA不存在于感染的细胞中。此时，在这些感染细胞中观察到的短暂细胞病变效应也消失了。少量未整合的线性病毒DNA持续存在于这些细胞中。非致细胞病变病毒感染的细胞不显示这种未整合的病毒DNA的瞬时积累。用致细胞病变病毒感染并随后在中和抗体存在下生长的细胞未显示未整合的病毒DNA的瞬时积累或致细胞病变效应。这些结果证明了包膜亚群、未整合线性病毒DNA的瞬时积累和禽白血病病毒的瞬时细胞杀伤之间的相关性。细胞杀伤似乎是由致细胞病变的禽白血病病毒引起的大规模第二轮重复感染的结果。
Avian leukosis viruses of subgroups B, D, and F are cytopathic for chicken cells, whereas viruses of subgroups A, C, and E are not. The amounts of unintegrated linear viral DNA in cells at different times after infection with cytopathic or noncytopathic viruses were determined by hybridization and transfection assays. Shortly after infection, there is a transient accumulation of unintegrated linear viral DNA in cells infected with cytopathic avian leukosis viruses. By 10 days after infection, the majority of this unintegrated viral DNA is not present in the infected cells. The transient cytopathic effect seen in these infected cells also disappears by this time. Low amounts of unintegrated linear viral DNA persist in these cells. Cells infected with noncytopathic viruses do not show this transient accumulation of unintegrated viral DNA. Cells infected with cytopathic viruses and subsequently grown in the presence of neutralizing antibody do not show the transient accumulation of unintegrated viral DNA or cytopathic effects. These results demonstrate a correlation between envelope subgroup, transient accumulation of unintegrated linear viral DNA, and transient cell killing by avian leukosis viruses. The cell killing appears to be the result of massive second-round superinfection by the cytopathic avian leukosis viruses.