Diversity within the current algal species Prototheca zopfii:: a proposal for two Prototheca zopfii genotypes and description of a novel species, Prototheca blaschkeae sp nov.
Diversity within the current algal species Prototheca zopfii:: a proposal for two Prototheca zopfii genotypes and description of a novel species, Prototheca blaschkeae sp nov.
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DOI：
10.1099/ijs.0.63892-0
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发表时间：
2006-06-01
影响因子：
2.8
通讯作者：
Truyen, U
中科院分区：
文献类型：
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作者：
Roesler, U;Möller, A;Truyen, U
为了明确非光合藻类zoopfiprototheca zoopfii的种内分类地位，以及牛乳腺原苔病的病因，采用多相分子方法对从临床原苔性乳腺炎病例和不同环境生境中分离的zoopfip菌株进行了鉴定。基于对18S rRNA基因的序列分析，发现目前已知的三种生物型或“变体”之间存在明显差异，设计了特异性寡核苷酸并用于生物型特异性pcr。此外，还对细胞脂肪酸的分布规律进行了评价。这些技术的分型结果表明，先前确定的血吸虫生物型明显不同。基于序列分析、脂肪酸模式和生理特征，提出生物型3应重新归类为代表新种Prototheca blaschkeae sp. nov(型菌株，RZIII-3（T) = SAG 2064(T)）。此外，我们还提出，将棘孔拟虫重新分类为一个包含至少两个基因型的物种，在未来可能被认为代表两个亚种。
In order to clarify the intraspecies taxonomic position of the non-photosynthetic algal species Prototheca zopfii, as well as the aetiology of bovine mammary protothecosis, a selection of P. zopfii strains isolated from clinical cases of protothecal mastitis and from various environmental habitats was characterized using a polyphasic molecular approach. Based on sequence analysis of the 18S rRNA gene, which showed distinct differences between the three currently known biotypes or 'variants', specific oligonucleotides were designed and used in biotype-specific PCRs. Furthermore, the pattern of cellular fatty acids was evaluated. Typing by means of these techniques revealed that the previously defined biotypes of P. zopfii were clearly different. Based on sequence analysis, the pattern of fatty acids and physiological characteristics, it is proposed that biotype 3 should be reclassified as representing a novel species, Prototheca blaschkeae sp. nov. (type strain, RZIII-3(T) = SAG 2064(T)). Furthermore, it is proposed that P. zopfii merits reclassification as a species comprising at least two genotypes that in future could probably be considered to represent two subspecies.