Storage lipid synthesis is necessary for autophagy induced by nitrogen starvation
Storage lipid synthesis is necessary for autophagy induced by nitrogen starvation
复制标题
储存脂质合成对于氮饥饿诱导的自噬是必需的
DOI：
10.1016/j.febslet.2014.11.050
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发表时间：
2015-01-16
期刊：
影响因子：
3.5
通讯作者：
Xie, Zhiping
中科院分区：
文献类型：
--
作者：
Li, Dan;Song, Jing-Zhen;Xie, Zhiping
氮饥饿是自噬的普遍刺激因素。目前，人们对氮饥饿条件下碳代谢与自噬之间的关系知之甚少。在这里，我们表明酵母细胞在氮饥饿下继续消耗葡萄糖并下调发酵。储存脂质产量增加，同时脂滴增殖。此外，我们提供的证据表明三酰甘油合成对于自噬体生物发生至关重要。它参与 PAS（吞噬细胞组装位点）支架组装的下游步骤，以及 Atg1、Atg14、Atg5 和 Atg8 募集的上游步骤。最后，我们证明脂滴与含有 Atg8 的膜短暂地相互作用。我们的研究揭示了中性脂质代谢、脂滴和自噬之间的新联系。 (C) 2014 年欧洲生化学会联合会。由 Elsevier B.V. 出版。保留所有权利。
Nitrogen starvation is a universal stimulus of autophagy. At present, little is known about the relationship between carbon metabolism and autophagy under nitrogen starvation. Here, we show that yeast cells continue to consume glucose and downregulate fermentation under nitrogen starvation. Storage lipid production is increased, with concurrent proliferation of lipid droplets. Furthermore, we provide evidence that triacylglycerol synthesis is crucial for autophagosome biogenesis. It is involved in a step downstream of PAS (phagophore assembly site) scaffold assembly, and upstream of the recruitment of Atg1, Atg14, Atg5 and Atg8. Finally, we demonstrate that lipid droplets transiently interact with Atg8-containing membranes. Our study reveals a novel connection linking neutral lipid metabolism, lipid droplets and autophagy. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.