CXCL17, an orphan chemokine, acts as a novel angiogenic and anti-inflammatory factor
CXCL17, an orphan chemokine, acts as a novel angiogenic and anti-inflammatory factor
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DOI：
10.1152/ajpendo.00083.2012
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发表时间：
2013-01-01
影响因子：
5.1
通讯作者：
Luo, Ching-Wei
中科院分区：
文献类型：
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作者：
Lee, Wei-Yu;Wang, Chun-Jen;Luo, Ching-Wei
李伟英，王建君，林天艳，萧清良，罗庆华。CXCL 17是一种孤儿趋化因子，是一种新的血管生成和抗炎因子。Am J Physiol Endocrinol Metab 304：E32-E40，2013.首次发表于2012年10月31日; doi：10.1152/ajpendo.00083.2012.-在生理和病理条件下，趋化因子在各种免疫细胞向各种组织的募集中起关键作用。CXCL 17是一种孤儿趋化因子，初步发现参与肿瘤血管生成。然而，它的蛋白质性质，以及其内源性生物活性，尚未得到很好的澄清。使用实时PCR，免疫组化染色，和Western印迹，我们发现，CXCL 17是高度表达的组成和诱导的方式在大鼠胃粘膜，在那里它经历蛋白质成熟过程中的内蛋白水解。成熟的CXCL 17表现出强大的趋化能力，靶向单核细胞和巨噬细胞，可能通过ERK 1/2和p38，而不是JNK信号转导。CXCL 17还诱导处理的单核细胞产生促血管生成因子，如血管内皮生长因子A。此外，与其他加速炎症反应的CXC趋化因子相比，CXCL 17对LPS激活的巨噬细胞显示出新的抗炎作用。因此，我们的数据表明，胃固有层中的CXCL 17可能在组织修复和抗炎中发挥重要作用，这两者都有助于维持胃粘膜的完整性。
Lee WY, Wang CJ, Lin TY, Hsiao CL, Luo CW. CXCL17, an orphan chemokine, acts as a novel angiogenic and anti-inflammatory factor. Am J Physiol Endocrinol Metab 304:E32-E40, 2013. First published October 31, 2012; doi:10.1152/ajpendo.00083.2012.-Chemokines play pivotal roles in the recruitment of various immune cells to diverse tissues in both physiological and pathological conditions. CXCL17 is an orphan chemokine preliminarily found to be involved in tumor angiogenesis. However, its protein nature, as well as its endogenous bioactivity, has not been well clarified. Using real-time PCR, immunohistochemical staining, and Western blotting, we found that CXCL17 is highly expressed in both a constitutive and inducible manner in the rat gastric mucosa, where it undergoes endoproteolysis during protein maturation. The mature CXCL17 exhibited strong chemoattractant abilities targeting monocytes and macrophages, potentially through ERK1/2 and p38 but not JNK signaling. CXCL17 also induced the production of proangiogenic factors such as vascular endothelial growth factor A from treated monocytes. Furthermore, in contrast to other CXC chemokines that accelerate inflammatory responses, CXCL17 showed novel anti-inflammatory effects on LPS-activated macrophages. Therefore, our data suggest that CXCL17 in the gastric lamina propria may play an important role in tissue repair and anti-inflammation, both of which help to maintain the integrity of the gastric mucosa.