Transcription factor fork head regulates the promoter of diapause hormone gene in the cotton bollworm, Helicoverpa armigera, and the modification of SUMOylation
Transcription factor fork head regulates the promoter of diapause hormone gene in the cotton bollworm, Helicoverpa armigera, and the modification of SUMOylation
复制标题
转录因子叉头调控棉铃虫滞育激素基因启动子及SUMO化修饰
DOI：
10.1016/j.ibmb.2011.04.009
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发表时间：
2011-09-01
影响因子：
3.8
通讯作者：
Xu, Wei-Hua
中科院分区：
文献类型：
--
作者：
Bao, Bin;Hong, Bo;Xu, Wei-Hua
转录因子叉头（FoxA）在发育和代谢中起重要作用。本研究克隆了棉铃虫叉头基因，发现叉头蛋白主要定位于细胞核中。该叉头基因属于Fox转录因子的FoxA亚家族。滞育激素和信息素生物合成激活神经肽（DH-PBAN）是两种已知的调节昆虫发育和信息素生物合成的神经肽，由同一mRNA编码。在本研究中，叉头被证明与H.棉铃虫DH-PBAN基因，并调控其启动子活性。进一步研究了FH蛋白SUMO化对Har-DH-PBAN基因表达调控的影响，结果表明SUMO修饰能够修饰Har-FH蛋白并导致DH-PBAN基因表达下调。这些结果表明SUMO化FH在H. armigera。(C)2011爱思唯尔有限公司版权所有。
The transcription factor fork head (FoxA) plays important roles in development and metabolism. Here, we cloned a fork head gene in Helicoverpa armigera, and found that the fork head protein is mainly located in the nucleus. This fork head gene belongs to the FoxA subfamily of the Fox transcription factors. The diapause hormone and pheromone biosynthesis-activating neuropeptide (DH-PBAN), which are two well-documented insect neuropeptides that regulate insect development and pheromone biosynthesis, are encoded by a single mRNA. In the present study, fork head was shown to bind strongly to the promoter of H. armigera DH-PBAN gene, and regulate its promoter activity. Furthermore, the effect of SUMOylation of the FH protein on the regulation of Har-DH-PBAN gene was investigated, and we show that the SUMO can modify Har-FH protein and cause down-regulation of DH-PBAN gene expression. These results suggest that SUMOylated FH plays a key role in insect diapause in H. armigera. (C) 2011 Elsevier Ltd. All rights reserved.