Isolation and characterization of a Vibrio cholerae gene (tagA) that encodes a ToxR-regulated lipoprotein.
Isolation and characterization of a Vibrio cholerae gene (tagA) that encodes a ToxR-regulated lipoprotein.
复制标题
编码 ToxR 调节脂蛋白的霍乱弧菌基因 (tagA) 的分离和表征。
DOI：
10.1016/0378-1119(94)00811-6
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发表时间：
1995
期刊：
影响因子：
3.5
通讯作者：
Peterson,KM
中科院分区：
文献类型：
--
作者：
Harkey,CW;Everiss,KD;Peterson,KM
霍乱弧菌(Vc)编码TagA脂蛋白的基因(TagA)已被分离。TagA序列分析表明，TagA有一个由568个氨基酸组成的开放阅读框(ORF)，在N端有一个特征的信号肽酶II裂解位点。T7启动子/RNA聚合酶诱导表达TagA后，对大肠杆菌(EC)细胞合成的蛋白质进行了电泳分析，发现与两种TagA相对应的一对紧密迁移的蛋白质。计算机生成的比对算法预测VC Taga和EC K99菌毛生物发生决定簇之间存在同源性。
The Vibrio cholerae (Vc) gene (tagA) coding for the TagA lipoprotein has been isolated. Sequencing of tagA revealed the presence of an open reading frame (ORF) of 568 amino acids with a characteristic signal peptidase II cleavage site at the N terminus. Electrophoretic analysis of proteins synthesized by Escherichia coli (Ec) cells following T7 promoter/RNA polymerase-directed expression of tagA, revealed a closely migrating doublet of proteins corresponding to two species of TagA. Computer-generated alignment algorithms predict that a homology exists between Vc TagA and Ec K99 fimbriae biogenesis determinant FanD.